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Enterococcus faecium microcapsule formulation and method for preparing same

An Enterococcus faecium and microcapsule technology, applied in the field of microorganisms, can solve the problems of limited large-scale application, large loss of active ingredients, short product shelf life, etc. Effect

Inactive Publication Date: 2011-08-10
ACAD OF STATE GRAIN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Enterococcus faecium does not form spores during the growth process, and has poor stress resistance, so it is difficult to store for a long time in liquid conditions
[0004] In practical application, when it is added and used in compound pellet feed, it is often subjected to high-temperature heating and granulation treatment, resulting in a large loss of active ingredients in the product and a short shelf life of the product, which limits its large-scale application

Method used

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  • Enterococcus faecium microcapsule formulation and method for preparing same
  • Enterococcus faecium microcapsule formulation and method for preparing same
  • Enterococcus faecium microcapsule formulation and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Screening of Enterococcus faecium and preparation of seed solution

[0032] 1. Method:

[0033] Feces from newborn infants were obtained by aseptic operation, diluted to a certain number of times, spread on a plate made of a specific medium, and cultured for 24 hours to obtain a certain number of colonies, and each colony was regarded as a selected target strain. After the growth curve, metabolic process determination and pathogenic bacteria inhibition experiment, the strains with high growth vigor and lactic acid production and significant antibacterial effect were selected as the final target strains and seed strains for coating.

[0034] 2. Enterococcus faecium culture and its culture conditions:

[0035] The optimal culture medium for Enterococcus faecium screened by single factor and orthogonal experiment is: glucose 10g / l, soybean peptone 5g / l, yeast extract 2g / l, ammonium citrate 2g / l, sodium acetate 5g / l , K2HPO 4 2g / l, Tween 801ml, MgSO 4 ·7H 2...

Embodiment 2

[0038] Embodiment 2: biological activity experiment

[0039] 1. Method

[0040] The growth, metabolism and bacteriostasis of Enterococcus faecium isolated in this laboratory were compared with those of imported Enterococcus faecalis and Enterococcus faecalis.

[0041] in:

[0042] MLK: Enterococcos from improt probiotics, reference strain, screened from Micola cream in the United States.

[0043] Baby-1: Enterococcos faecalis (Obtaied by our lab), Enterococcus faecalis Baby-1.

[0044] Baby-2: Enterococcos faecium, Enterococcus faecalis Baby-2.

[0045] 1. Comparison of growth, metabolism and antibacterial conditions:

[0046] Activate two generations of tested strains with 1% inoculum size (about 2×10 6 cfu / mL) were inoculated in a 300mL Erlenmeyer flask containing 100mL of MRS medium, cultured at 37°C and 150rpm, samples were taken every 2 hours, and the absorbance at 600nm was measured. Take the culture time as the abscissa and the corresponding absorbance value as th...

Embodiment 3

[0056] Embodiment 3: preparation microcapsule preparation

[0057] 1. Materials:

[0058] The capsule material is sodium alginate solution with a concentration of 1.5g / l (1.5%w / v).

[0059] The curing agent is calcium chloride (CaCl 2 ) aqueous solution with a concentration of 0.1 mol / l.

[0060] Enterococcus faecium, the preservation number is CGMCC No.2516.

[0061] 2. Microencapsulation process of Enterococcus faecium:

[0062] 1. Preparation and sterilization of capsule material solution: Add sodium alginate to distilled water in batches under continuous stirring, stir to make a transparent glue solution, sterilize at 115°C for 20 minutes, and set aside.

[0063] 2. Preparation and sterilization of curing agent: make a 0.1mol / l solution with distilled water, sterilize at 115°C for 20 minutes, and set aside.

[0064] 3. Enterococcus faecium seed liquid culture: using the culture medium and culture conditions described in Example 1, the bacteria in the logarithmic growt...

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Abstract

The present invention discloses micro-capsule preparation and a method for preparing the same, belonging to the microorganism field and the preparation field. The micro-capsule preparation uses sodium alginate as capsule material solution and CaCl2 as curing agent and contains Enterococcus Faecium the preservation number of which is CGMCC No.2516. The invention has the advantages that: the pre-embedding and after fermentation method is adopted, thereby thalli can be proliferated continuously under the micro-capsule microenvironment; the size of the cell density is controllable; the density of the micro-capsule thalli higher than that of the after embedding method can be reached; the pre-embedding method greatly reduces the utilization amount of cells during the encapsulization process, avoids the adverse factors of low encapsulization efficiency and productive rate due to impact of the cell density in the after embedding process, and more easily obtains encapsulated products with better shape and stability.

Description

technical field [0001] The invention relates to an Enterococcus faecium microcapsule preparation and a preparation method thereof, more specifically a highly active Enterococcus faecium (Enterococcus Faecium) and a new microcapsule formulation thereof, belonging to the field of microorganisms. Background technique [0002] Enterococcus faecium, as an indigenous flora in the intestinal tract of livestock and poultry, has a significant effect on improving the growth performance of livestock and poultry. [0003] Enterococcus faecium does not form spores during the growth process, and has poor stress resistance, so it is difficult to preserve for a long time under liquid conditions. [0004] In practical applications, when it is added to compound pellet feed, it often needs to be heated and granulated at high temperature, resulting in a large loss of active ingredients in the product and a short shelf life of the product, which limits its large-scale application. Probiotics ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23P1/04C12R1/01A23K10/18A23K40/30
Inventor 綦文涛郭蕴劼郝淑红张晓琳郭伟群宋渊
Owner ACAD OF STATE GRAIN ADMINISTRATION
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