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Reagent kit for detecting adiposity genetic susceptibility

A kit, susceptibility technology, used in molecular biology and medicine, to solve problems such as the failure to determine the mode of inheritance

Inactive Publication Date: 2009-01-28
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The occurrence of obesity is the result of energy metabolism disorders in the body. Its exact pathogenesis is not yet fully understood. At present, it is believed that in addition to environmental factors and living habits, obesity is also related to genetics, central nervous system abnormalities, endocrine dysfunction, and metabolic factors. It is related to the imbalance of nutritional factors, etc. People have long noticed the family tendency of obesity and a strong genetic background, but they have not been able to determine the mode of inheritance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Use of detection kits

[0023] Step 1: Extraction of DNA template

[0024] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0025] Step 2: Real-time quantitative PCR reaction

[0026] Use a fluorescent quantitative PCR detection kit for individual genetic susceptibility to antioxidant, which contains the following primer pairs and fluorescent probe pairs:

[0027] Sense primer 1: 5'-CAGACCAGGAGCTGATCTGCTT-3' (SEQ ID NO: 1)

[0028] Antisense primer 1: 5'-AGTCCCAGACATTGCAGTTGAAG-3' (SEQ ID NO: 2)

[0029] Sense primer 2: 5'-AGGACGATGAGAGACATGACGAT-3' (SEQ ID NO: 3)

[0030] Antisense primer 2: 5'-AACGGCAGCGCCTTCTT-3' (SEQ ID NO: 4)

[0031] Probe 1 with VIC fluorophore: 5'-TCACGTCcGTGGCC-3' (SEQ ID NO: 5)

[0032] Probe 1 with FAM fluorophore: 5'-ATCACGTCtGTGGCCT-3' (SEQ ID NO: 6)

[0033] Probe 2 with VIC fluorophore: 5'-CCCTTTgCTGCGTGACGTCGT-3' (SEQ ID NO: 7)

[0034] Probe 2 with FAM fluorophore: 5'-TCCCTTTcCTGCGTGACGTC...

Embodiment 2

[0041] Example 2. Services for Testing People for Genetic Susceptibility to Obesity

[0042] Step 1: DNA Extraction

[0043] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0044] Step 2: Genotyping Assays

[0045] Using the kit provided by the present invention, the rs5443 SNP site on the GNB3 gene of the subject's genomic DNA and the rs1042714 SNP site on the ADRB2 gene are respectively subjected to fluorescent quantitative PCR detection to determine the genes of the two SNP sites type.

[0046] Step 3: Analysis of genetic susceptibility to obesity

[0047]Through the analysis of the SNP genotype of the tested subjects, an analysis report of the genetic susceptibility to obesity is issued. The report specifies the level of the genetic susceptibility of the subject to obesity in detail, and the ph...

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PUM

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Abstract

The invention discloses a kit that is used for detecting the genetic predisposition of adiposity. The kit comprises particularity primer pairs and particularity fluorescent probe pairs that are used for simultaneously detecting rs5443 SNP locus on the gene of GNB3 and rs1042714 SNP locus on the gene of ADRB2 and a routine component that is used for fluorescent quantitative PCR detection, etc. The kit of the invention evaluates the genetic predisposition of individual adiposity by simultaneously detecting the mononucleotide polymorphism locus genotypes on the genes of GNB3 and ADRB2 that are closely related with the genetic predisposition of adiposity.

Description

technical field [0001] The present invention relates to the field of molecular biology and medicine, more specifically, the present invention relates to a kind of test kit of detecting genetic susceptibility to obesity, by simultaneously detecting the encoding G protein β3 subunit (G Protein beta polypeptide 3, GNB3) and β2-adrenergic receptor gene (β2-adrenergic receptor, β2-AR, ADRB2) genotypes of single nucleotide polymorphisms on two genes to assess genetic susceptibility to obesity . Background technique [0002] Obesity is a chronic disease closely related to genetic factors and lifestyle. It can cause elevated blood lipids, which can easily lead to fatty liver, arterial vascular disease, heart disease, diabetes, and obese children can also cause sexual dysfunction, etc. . This not only poses a serious threat to health in childhood, but also is an important reason for the increase in morbidity and mortality of various diseases in adulthood. Among them, childhood obe...

Claims

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Application Information

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IPC IPC(8): G01N21/00G01N33/50C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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