Germplasm identification method for moerella irideseens

Abstract

The invention relates to a germplasm identification method for Moerella iridescens, which is characterized by comprising the following steps: at least 30 Moerella iridescens samples to be measured are taken to undergo determination of 7 morphologic parameters, and the 7 morphologic parameters, namely the shell length CD of the Moerella iridescens, the maximum distance AF from the shell top to theanterior adductor muscle scar, the length IJ of the anterior border and the posterior border of a pallial scar, the length AE from the shell top to the posterior border of the pallial scar, the shellheight AB, the shell width SW and the distance GH between the anterior adductor muscle scar and the posterior adductor muscle scar are determined respectively; the parameter value of the shell lengthCD is taken as a denominator and the residual 6 parameter values are taken as numerators, and the following 6 proportion parameter vales are obtained: XAF:AF / CD, XIJ:IJ / CD, XAE:AE / CL, XAB:AB / CL, XSW:SW / CD and XGH:GH / CD; and the 6 proportion parameter values are substituted into a discriminant equation for result determination respectively, and a colony wherein the occurrence frequency of the maximum of the Y value obtained is maximum is taken as a source of the Moerella iridescens samples to be measured. The method can quickly, economically and relatively accurately identify the germplasm of the Moerella iridescens in main production places of China with simple and convenient operation.

Description

technical field The invention relates to a germplasm identification method, in particular to a germplasm identification method of the rainbow clam. Background technique Rainbow bright cherry clam (Moerella iridescens Benson) belongs to the mollusk phylum, petal branch class, tellinidae family (Tellinidae), commonly known as plum clam, yellow clam, sea melon seeds, razor clam. Rainbow clams are naturally distributed in various sea areas of my country, and the main resource area is the tidal flat of Hangzhou Bay in the East China Sea. Therefore, this clam has become an important small economic shellfish naturally caught in Jiangsu, Zhejiang and Shanghai, and its price has been rising year by year. Driven by high profits, coastal fishermen have over-harvested and raised shrimps on the beach, which has seriously damaged the natural ecology, and its resources have declined sharply. Due to trade liberalization, natural seedlings in various sea areas have been harvested and artific...

AI Technical Summary

Problems solved by technology

At present, the molecular markers commonly used to evaluate biological genetic diversity and germplasm identification have a certain degree of defects, such as RAPD technology (Random amplified polymorphic DNA, Random amplified polymorphic DNA), often because RAPD primers are too short by 10 bases The base and annealing temperature are too low, generally 36-37°C, leading to some non-specific results of the amplified product, and its reliability and authenticity are the main defects; and AFLP technology (amplified fragment length polymorphism, Amplified fragment length polymorphism) , and its wide application is limited due to complex operating conditions such as enzyme digestion and relatively high cost. Microsatellite technology is difficult to screen for molecular markers and requires advanced technology and equipment to limit its wide application.

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