Method for producing concentrated inactivate vaccine for newcastle disease
A technology of chicken Newcastle disease and production method, which can be applied in the field of bioengineering and can solve the problems of granuloma, scattered toxin, malabsorption and the like
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Embodiment 1
[0028] Chicken Newcastle disease virus attenuated LaSota strain was used as a virus seed to inoculate 10-day-old SPF chicken embryos, and the chicken embryo fluid that died 72-120 hours after inoculation and had obvious lesions was harvested. After concentration, formalin solution was added to make the content reach 0.2%. Inactivated at 37°C for 16 hours after shaking. Before inactivation, the virus content was determined according to the ministerial standard, and the virus content was more than 10 8.8 EID 50 / 0.1ml. The inactivated virus solution was inoculated into 6 SPF chicken embryos, and after 5 days of observation, no more than one chicken embryo died non-specifically. The hemagglutination value of all embryo fluids was measured separately, and it was used when no hemagglutination occurred. Add sterile 5% Tween-80 to the inactivated venom to form an aqueous phase; add 6% Span-80 to No. 10 white oil, and then add 2% aluminum stearate to form an oil phase after steril...
Embodiment 2
[0031] Chicken Newcastle disease virus attenuated LaSota strain was inoculated with 10-day-old SPF chicken embryos, and the chicken embryo liquid that died 72-120 hours after inoculation and had obvious lesions was harvested. The NDV chicken embryo liquid was concentrated by using a Milipore ultrafiltration membrane with a cut-off molecular weight of 30KD. Add formalin solution to make the content 0.2%. Inactivated at 37°C for 16 hours after shaking. The inactivated virus liquid is tested for inactivation, and the completely inactivated venom is ready for use. Add sterile 5% Tween-80 to the inactivated venom to form an aqueous phase; add 6% Span-80 to No. 10 white oil, and then add 2% aluminum stearate to form an oil phase after sterilization. Mix and emulsify the water phase and the oil phase at a ratio of 1:1 in a high-pressure emulsifying pump, that is, the concentrated and inactivated vaccine of Newcastle disease in chickens, with a virus content >108.8 EID 50 / 0.1ml.
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