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Method for producing concentrated inactivate vaccine for newcastle disease

A technology of chicken Newcastle disease and production method, which can be applied in the field of bioengineering and can solve the problems of granuloma, scattered toxin, malabsorption and the like

Active Publication Date: 2011-08-31
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, although the use of vaccines has effectively controlled the disease, due to the unsatisfactory immune effect of the vaccine, the enhanced virulence of the epidemic strains and the uneven level of my country's chicken industry, the disease is still a serious threat to my country's chicken industry. One of the diseases of the poultry industry, atypical Newcastle disease often occurs in my country, and even the outbreak of typical Newcastle disease
[0009] At present, the vaccines used to prevent and control the disease mainly include live vaccines and inactivated vaccines. Among them, live vaccines include attenuated vaccines II, III, IV and moderately virulent vaccines I [2]. The disadvantage of attenuated vaccines is that they cannot break through Interference from maternal antibodies, although moderately virulent vaccines can break through the influence of maternal antibodies and produce antibodies quickly, they can cause chickens to become sick and have the risk of spreading the virus. It is reported that they can cause atypical Newcastle disease in chickens
Inactivated vaccines are mainly oil emulsion inactivated vaccines, which can avoid the influence of maternal antibodies and have no risk of spreading the virus, and are suitable for immunization of chickens of any age; The dose is constantly increasing, which brings inconvenience to immunization, and the side effects of vaccine immunization are enhanced, and malabsorption or granuloma often occurs at the inoculation site, which affects the quality of poultry products and causes certain losses to the poultry industry.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0028] Chicken Newcastle disease virus attenuated LaSota strain was used as a virus seed to inoculate 10-day-old SPF chicken embryos, and the chicken embryo fluid that died 72-120 hours after inoculation and had obvious lesions was harvested. After concentration, formalin solution was added to make the content reach 0.2%. Inactivated at 37°C for 16 hours after shaking. Before inactivation, the virus content was determined according to the ministerial standard, and the virus content was more than 10 8.8 EID 50 / 0.1ml. The inactivated virus solution was inoculated into 6 SPF chicken embryos, and after 5 days of observation, no more than one chicken embryo died non-specifically. The hemagglutination value of all embryo fluids was measured separately, and it was used when no hemagglutination occurred. Add sterile 5% Tween-80 to the inactivated venom to form an aqueous phase; add 6% Span-80 to No. 10 white oil, and then add 2% aluminum stearate to form an oil phase after steril...

Embodiment 2

[0031] Chicken Newcastle disease virus attenuated LaSota strain was inoculated with 10-day-old SPF chicken embryos, and the chicken embryo liquid that died 72-120 hours after inoculation and had obvious lesions was harvested. The NDV chicken embryo liquid was concentrated by using a Milipore ultrafiltration membrane with a cut-off molecular weight of 30KD. Add formalin solution to make the content 0.2%. Inactivated at 37°C for 16 hours after shaking. The inactivated virus liquid is tested for inactivation, and the completely inactivated venom is ready for use. Add sterile 5% Tween-80 to the inactivated venom to form an aqueous phase; add 6% Span-80 to No. 10 white oil, and then add 2% aluminum stearate to form an oil phase after sterilization. Mix and emulsify the water phase and the oil phase at a ratio of 1:1 in a high-pressure emulsifying pump, that is, the concentrated and inactivated vaccine of Newcastle disease in chickens, with a virus content >108.8 EID 50 / 0.1ml.

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Abstract

The invention provides a method for producing improved concentrated inactivated vaccine against Newcastle disease. The method comprises the steps of virus inoculation passage, ultrafiltration and concentration, mixing water phase and oil phase and the like, wherein, a 30KD membrane is used for ultra-filtrating and concentrating, and the concentrated water phase and oil phase are mixed and emulsified in the proportion of 1 to 1 in a high pressure emulsification pump. The invention uses ultrafilter membrane concentration and antigen ingredient instead of the content of live virus, so that the chicken can produce strong immunity after being immunized by the vaccine for 10 to 14 days; the immunity period is 10 months; the anti-virus protective rate is 100 percent within the immunity period; and the invention has wide application prospect.

Description

technical field [0001] The present invention relates to the improvement of a vaccine production technology, in particular, to an improved method for the concentration technology of concentrated and inactivated chicken Newcastle disease vaccine. It belongs to the field of bioengineering. Background technique [0002] Newcastle disease virus (NDV) is also known as Asian chicken plague virus, pseudofowl plague virus or avian pneumonia virus. It belongs to a species in the family Paramyxoviridae and the genus Paramyxovirus in the taxonomy of viruses. The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly in infected flocks, and the virulent strain can destroy the entire flock. Attenuated strains cause only respiratory infections and reduced egg production in flocks, but recover quickly. Humans can develop conjunctivitis or lymphadenitis from exposure to sick poultry and live vaccines, but recover quickly. Newcastle disease virus is designated by OIE a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/17A61P31/14
Inventor 熊蕊杨保收
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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