Method for producing concentrated inactivate vaccine for newcastle disease

Abstract

The invention provides a method for producing improved concentrated inactivated vaccine against Newcastle disease. The method comprises the steps of virus inoculation passage, ultrafiltration and concentration, mixing water phase and oil phase and the like, wherein, a 30KD membrane is used for ultra-filtrating and concentrating, and the concentrated water phase and oil phase are mixed and emulsified in the proportion of 1 to 1 in a high pressure emulsification pump. The invention uses ultrafilter membrane concentration and antigen ingredient instead of the content of live virus, so that the chicken can produce strong immunity after being immunized by the vaccine for 10 to 14 days; the immunity period is 10 months; the anti-virus protective rate is 100 percent within the immunity period; and the invention has wide application prospect.

Description

technical field [0001] The invention relates to an improvement of a vaccine production technology, in particular to an improvement method of a concentrated inactivated Newcastle disease vaccine concentration technology. Belongs to the field of bioengineering. Background technique [0002] Newcastle disease virus (Newcastlediseasevirus, NDV) is also known as Asian fowl plague virus, pseudo fowl plague virus or avian pneumoencephalitis virus. The position in virus taxonomy belongs to Paramyxoviridae (Paramyxoviridae), a species in Paramyxovirus genus (Paramyxovirus). The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly among the infested flocks, and the virulent strain can destroy the whole flock of chickens. Attenuated strains only cause respiratory tract infection and decreased egg production in chickens, but they can recover quickly. Humans can develop conjunctivitis or lymphadenitis due to contact with sick birds and live virus vaccines, but re...

AI Technical Summary

Problems solved by technology

[0008] At present, although the use of vaccines has effectively controlled the disease, due to the unsatisfactory immune effect of the vaccine, the enhanced virulence of the epidemic strains and the uneven level of my country's chicken industry, the disease is still a serious threat to my country's chicken industry. One of the diseases of the poultry industry, atypical Newcastle disease often occurs in my country, and even the outbreak of typical Newcastle disease

[0009] At present, the vaccines used to prevent and control the disease mainly include live vaccines and inactivated vaccines. Among them, live vaccines include attenuated vaccines II, III, IV and moderately virulent vaccines I [2]. The disadvantage of attenuated vaccines is that they cannot break through Interference from maternal antibodies, although moderately virulent vaccines can break through the influence of maternal antibodies and produce antibodies quickly, they can cause chickens to become sick and have the risk of spreading the virus. It is reported that they can cause atypical Newcastle disease in chickens

Inactivated vaccines are mainly oil emulsion inactivated vaccines, which can avoid the influence of maternal antibodies and have no risk of spreading the virus, and are suitable for immunization of chickens of any age; The dose is constantly increasing, which brings inconvenience to immunization, and the side effects of vaccine immunization are enhanced, and malabsorption or granuloma often occurs at the inoculation site, which affects the quality of poultry products and causes certain losses to the poultry industry.