Method for improving tumor cell cloning efficiency

Abstract

The invention discloses a method for improving tumor cell cloning efficiency; firstly, tumor stem cells are separated out from primary tumor cells, single tumor stem cell is adopted to carry out cellclone, the cloning efficiency of the primary tumor cells is greatly improved by utilizing the biological characteristics of the tumor stem cells, great convenience is provided for lone building, purification and late research of the primary tumor cells, and the method has good application prospect.

Description

technical field [0001] The invention relates to a method for cloning tumor cells, in particular to a method for increasing the formation rate of tumor cell clones. Background technique [0002] The traditional tumor cell cloning method usually adopts the limiting dilution method. However, due to the different proliferation ability and growth characteristics of each tumor cell, the number of cells that can finally form clones is limited, and the clone formation rate is extremely low. A large number of studies have shown that tumor cells will form a certain number of cell spheres under the conditions of stem cell culture medium, and most of these cell spheres are tumor stem cells. Compared with differentiated tumor cells, cancer stem cells have special biological characteristics, that is, they have the ability of self-renewal, unlimited proliferation and multilineage differentiation. Therefore, the rate of tumor cell clone formation is directly related to tumor stem cells. ...

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Problems solved by technology

[0002] The traditional tumor cell cloning method usually adopts the limiting dilution method, but due to the different proliferation ability and gr

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