Method for fast measuring threonine content

A technique for rapid determination of threonine, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials. It can solve the problem of difficult industrial production guidance, long measurement time and high accuracy Low problems, to achieve the effect of fast measurement method, high accuracy, and improved accuracy

Active Publication Date: 2011-10-05
NINGXIA EPPEN BIOTECH
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  • Application Information

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Problems solved by technology

However, the measurement time of paper chromatography is about 3-4 hours each time, and the accuracy rate is very low, and the error value can reach about 10%, and due to the long measurement time, it is difficult to carry out re-testing, so it is difficult to carry out industrial production. direct guidance

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: A method for rapidly detecting the content of threonine, the method includes the following steps: prepare standard threonine solutions of 0.20g / L, 0.25g / L, 0.30g / L, and 0.35g / L respectively according to needs , according to the estimated concentration of threonine in the sample, dilute the sample so that the diluted concentration is between 0.20g / L and 0.35g / L. Take 1ml of the standard threonine solution and the diluted sample solution and add them to a 20×200 test tube respectively, then add 2ml of 0.2mol / L citric acid-sodium citrate buffer solution with pH=6.00 and 1ml of ninhydrin to show Put the color solution in a four-hole water bath and heat at 96°C for 10 minutes, take it out and cool it to room temperature, add 10ml of distilled water and shake well, then measure the absorbance with a wavelength of 530nm under a spectrophotometer. Draw a standard curve according to the standard threonine solution concentration and the measured absorbance results, ...

Embodiment 2

[0021] Embodiment 2: A method for rapidly detecting the content of threonine, the method comprises the following steps: prepare standard threonine solutions of 0.20g / L, 0.25g / L, 0.30g / L, and 0.35g / L respectively as required , according to the estimated concentration of threonine in the sample, dilute the sample so that the diluted concentration is between 0.20g / L and 0.35g / L. Take 1ml of the standard threonine solution and the diluted sample solution and add them to a 20×200 test tube respectively, then add 2ml of 0.2mol / L citric acid-sodium citrate buffer solution with pH=6.00 and 1ml of ninhydrin to show Put the color solution in a four-hole water bath and heat at 96°C for 8 minutes, take it out and cool it to room temperature, add 10ml of distilled water and shake well, then measure the absorbance with a wavelength of 540nm under a spectrophotometer. Draw a standard curve according to the standard threonine solution concentration and the measured absorbance results, and the...

Embodiment 3

[0022] Embodiment 3: A method for rapidly detecting the content of threonine, the method comprises the following steps: prepare standard threonine solutions of 0.10g / L, 0.20g / L, 0.30g / L, and 0.40g / L respectively as required , according to the estimated concentration of threonine in the sample, dilute the sample so that the diluted concentration is between 0.10g / L and 0.40g / L. Take 1ml of the standard threonine solution and the diluted sample solution and add them to a 20×200 test tube respectively, then add 2ml of 0.2mol / L citric acid-sodium citrate buffer solution with pH=6.00 and 1ml of ninhydrin to show Put the color solution in a four-hole water bath and heat at 97°C for 8 minutes, take it out and cool it to room temperature, add 10ml of distilled water and shake well, then measure the absorbance with a wavelength of 530nm under a spectrophotometer. Draw a standard curve according to the standard threonine solution concentration and the measured absorbance results, and the...

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Abstract

The invention relates to a method for detecting threonine content in production process sample in the biological fermentation engineering technical field, in particular to a method for fast measuring threonine content. The method comprises the following steps: preparing standard threonine solutions of different concentrations, then respectively taking standard threonine solutions and sample solution of certain amount, adding in buffer solution and developing solution respectively, water-bathing at certain temperature, using a spectrophotometer to perform colorimetric estimation to absorbancy,drawing standard curve according to the result of absorbancy estimation, and calculating the threonine content in the sample. The invention has the advantages of fastness, convenience and smaller error value with the measuring time controlled within one hour and the error within 2%.

Description

technical field [0001] The invention relates to a method for detecting the content of threonine in a production process sample in the technical field of biological fermentation engineering, in particular to a method for rapidly measuring the content of threonine. Background technique [0002] At present, high-performance liquid chromatography or amino acid analyzers can be used in the laboratory to detect the content of threonine in the solution, but because the instruments used in this method are expensive, it is difficult to popularize them. The commonly used method in the laboratory is paper chromatography, which does not require high equipment. Paper chromatography uses less equipment and is relatively convenient. However, the measurement time of paper chromatography is about 3-4 hours each time, and the accuracy rate is very low, and the error value can reach about 10%, and due to the long measurement time, it is difficult to carry out re-testing, so it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 马吉银刘多涛
Owner NINGXIA EPPEN BIOTECH
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