Method for screening corn salt/alkali-tolerance germplasm

A screening method and salt-alkali tolerance technology, applied in the field of crop cultivation, can solve the problems of high cost and long time consumption, and achieve the effect of low cost and short time consumption

Inactive Publication Date: 2010-07-28
JILIN ACAD OF AGRI SCI
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Problems solved by technology

[0004] At present, the saline-alkali tolerant germplasm screening of maize is mostly obtained by comparing the relative yield reduction rate after field production. This method takes a long time, requires a certain amount of land, and is expensive.

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  • Method for screening corn salt/alkali-tolerance germplasm
  • Method for screening corn salt/alkali-tolerance germplasm
  • Method for screening corn salt/alkali-tolerance germplasm

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example

[0017] A kind of corn saline-alkali tolerance germplasm screening technology, comprises the steps:

[0018] (1) Select 31 corn hybrids with consistent plumpness (see Table 1, wherein Zhengdan 958 and Xianyu 335 are control varieties), and use 0.1% HgCl 2 Disinfect for 10 minutes. A batch of seeds were in Na 2 CO 3 The seed germination test was carried out under the stress of the solution concentration of 25mmol / L and the NaCl solution concentration of 100mmol / L, using clear water as the control (CK), and repeated twice. 30 seeds were used for each stress condition, and the germination rate of the seeds was measured after 7 days of germination. Another batch of seeds were taken to germinate in the light incubator, and after the seeds germinated, the seeds were transplanted into plastic pots equipped with fine sand and cultivated in the greenhouse. The diurnal temperature of the growth chamber is (25±2)°C / (20±2)°C, the light is 12 hours a day, the light intensity is 2500-300...

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Abstract

The invention discloses a method for screening corn salt / alkali-tolerance germplasm, which belongs to the field of crop cultivation; the method comprises the following steps of: firstly putting a batch of corn seeds in a culture dish and testing the germination rate thereof; taking another batch of corn seeds for germination, culturing the seeds in a greenhouse, and stressing the seedlings when the seedlings grow to the three-leaf and one-core stage; taking the leaves of the seedlings to be tested, and testing the relative conductivity, the SOD activity, the MDA content, and the proline content; comparing the salt / alkali tolerances of the corn seeds. The method provided by the invention can complete the screening of the corn germplasm in a laboratory, in short time and with low cost.

Description

technical field [0001] The invention belongs to the field of crop cultivation, and specifically relates to a method for obtaining saline-alkali-tolerant maize germplasm based on detection and screening of physiological and biochemical indicators. Background technique [0002] Saline-alkali land is a type of soil widely distributed on the earth. It means that the salt contained in the soil affects the normal growth of crops. The acceleration of the global salinization process has aggravated the threat of salinity to human beings. It is one of the ecological crises faced by human beings. Its loss to agricultural production is second only to drought. According to incomplete statistics from UNESCO and FAO, the area of ​​saline-alkali land in the world is 954.38 million hectares, accounting for 10% of the arable land area, of which 99.13 million hectares is in my country, accounting for about 20% of the national arable land area . The western part of Jilin Province is an importa...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/02
Inventor 李晓辉张春宵刘文国李文华郝东云袁英刘晓鑫
Owner JILIN ACAD OF AGRI SCI
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