Aqueous two-phase system based on anion surface active agent, preparation method thereof

Abstract

The invention provides an aqueous two-phase system, a preparation method and application thereof. The aqueous two-phase system consists of 3-phenoxyacetic acid-2-hydroxypropyl trimethyl ammonium halide as an anion surface active agent, 1-butyl-3methylimidazolium tetrafluoroborate as ion liquid and water. The surface active agent in the system has small fraction mass, short phase forming time, high water content, simple system and moderate condition. The ion liquid and the anion surface active agent can be respectively recycled and have the advantages of low consumption and high efficiency andthe like. The aqueous two-phase system is a new extracting system with environment protection in the extraction and separation process and has good application prospect for the extraction and separation of the biological substances.

Description

technical field

[0001] The invention belongs to the frontier fields of colloid and interface chemistry, analytical chemistry and biochemistry, and especially relates to a new green solvent 1-butyl-3 methylimidazolium tetrafluoroborate ionic liquid and quaternary ammonium salt cationic surface active A new two-phase extraction system and a preparation method formed of agent 3-p-nonylphenoxy-2-hydroxypropyltrimethylammonium bromide and water. Background technique

[0002] Aqueous two-phase extraction technology is a new type of separation technology that has emerged in recent years. It uses the difference in the distribution of components between two aqueous phases to separate and purify components. This technology is widely used in biochemistry, cell biology, biochemical industry, food and In medicine and other fields, it is especially suitable for the separation and purification of active ingredients in biological substances and natural resources. Especially in recent years...

Examples

experiment example 1

[0035] Experimental Example 1 The cationic surfactant-based aqueous two-phase system obtained in Example 1 was used to extract and purify capsaicin. According to the total mass of the prepared two-phase aqueous system, take by weighing 3-p-nonylphenoxy-2-hydroxypropyltrimethylammonium halide, 26% ( g / g) of 1-butyl-3 methylimidazolium tetrafluoroborate and 66.0% double distilled water, shake and stir evenly with a turbo stirrer (Shanghai Jingke Instrument Co., Ltd.), and inject 5 to 20 μg of The oil-soluble sweet pepper pigment is injected into the two-phase aqueous phase, shaken and stirred, and placed at a constant temperature at a temperature lower than 27° C., and the solution is separated after 30 minutes. All the paprika pigments were extracted to the lower phase. This example proves that this system is an excellent extraction system for the separation and extraction of sweet pepper pigments.

[0036] Experimental Example 2 The cationic surfactant-based aqueous two-phas...

technical field

[0001] The invention belongs to the frontier fields of colloid and interface chemistry, analytical chemistry and biochemistry, and especially relates to a new green solvent 1-butyl-3 methylimidazolium tetrafluoroborate ionic liquid and quaternary ammonium salt cationic surface active A new two-phase extraction system and a preparation method formed of agent 3-p-nonylphenoxy-2-hydroxypropyltrimethylammonium bromide and water. Background technique

[0002] Aqueous two-phase extraction technology is a new type of separation technology that has emerged in recent years. It uses the difference in the distribution of components between two aqueous phases to separate and purify components. This technology is widely used in biochemistry, cell biology, biochemical industry, food and In medicine and other fields, it is especially suitable for the separation and purification of active ingredients in biological substances and natural resources. Especially in recent years...

Examples

experiment example 1

[0035] Experimental Example 1 The cationic surfactant-based aqueous two-phase system obtained in Example 1 was used to extract and purify capsaicin. According to the total mass of the prepared two-phase aqueous system, take by weighing 3-p-nonylphenoxy-2-hydroxypropyltrimethylammonium halide, 26% ( g / g) of 1-butyl-3 methylimidazolium tetrafluoroborate and 66.0% double distilled water, shake and stir evenly with a turbo stirrer (Shanghai Jingke Instrument Co., Ltd.), and inject 5 to 20 μg of The oil-soluble sweet pepper pigment is injected into the two-phase aqueous phase, shaken and stirred, and placed at a constant temperature at a temperature lower than 27° C., and the solution is separated after 30 minutes. All the paprika pigments were extracted to the lower phase. This example proves that this system is an excellent extraction system for the separation and extraction of sweet pepper pigments.

[0036] Experimental Example 2 The cationic surfactant-based aqueous two-phas...

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