Two aqueous phase system formed based on anionic surfactant and preparation method thereof

Abstract

The invention provides a two aqueous phase system formed based on anionic surfactant and a preparation technique and application thereof. The two aqueous phase system is composed of anionic surfactant sodium dodecyl benzene sulfonate, ionic liquid 1-butyl-3methyl imidazole tetrafluoroborate and water. The surfactant used in the system has small mass fraction, both the ionic liquid and the surfactant can be recycled, and the system is short in phasing time, high in water content, simple in structure, mild in phasing conditions, low in consumption and high in efficiency, is a new environment-friendly extraction system in extraction and separation system, and has a good application prospect especially in extraction and separation of biological substances.

Description

Technical field

[0001] The invention belongs to the frontier field of colloid and interface chemistry, analytical chemistry, and biochemistry, and particularly relates to a new green solvent 1-butyl-3 methylimidazole tetrafluoroborate ionic liquid and anionic surfactant 12 A new two-phase extraction system formed by sodium alkylbenzene sulfonate and water and its preparation method. Background technique

[0002] Aqueous two-phase extraction technology is a new type of separation technology that has emerged in recent years. It uses the difference in the distribution of components between two aqueous phases to separate and purify components. This technology is widely used in biochemistry, cell biology, biochemical industry, food industry, etc. due to its simple operation, low energy consumption, high separation efficiency, no damage and inactivation of the separated substances, easy engineering amplification and continuous operation, etc. Medicine and other fields, it is especiall...

Examples

experiment example 1

[0034] Experimental Example 1 The aqueous two-phase system based on anionic surfactants obtained in Example 1 was used to extract and purify biologically active substances. According to the total mass of the two-phase aqueous system, 1.0% (g / g) of sodium dodecylbenzene sulfonate and 39% (g / g) of 1-butyl-3 are weighed with a micro electronic balance. Methylimidazole tetrafluoroborate and 60.0% double distilled water, shake and stir evenly with a turbo agitator (Shanghai Jingke Instrument Co., Ltd.), use a micro syringe to inject 5-20μg of oil-soluble bell pepper pigment into the double water In the phase, shake, stir, and place at a constant temperature below 48°C. After 30 minutes, the solution separates. All capsicum pigments are extracted to the upper phase. This example proves that this system is an excellent extraction system for the separation and extraction of pepper pigments.

[0035] Experimental Example 2 The aqueous two-phase system formed based on anionic surfactants...

Technical field

[0001] The invention belongs to the frontier field of colloid and interface chemistry, analytical chemistry, and biochemistry, and particularly relates to a new green solvent 1-butyl-3 methylimidazole tetrafluoroborate ionic liquid and anionic surfactant 12 A new two-phase extraction system formed by sodium alkylbenzene sulfonate and water and its preparation method. Background technique

[0002] Aqueous two-phase extraction technology is a new type of separation technology that has emerged in recent years. It uses the difference in the distribution of components between two aqueous phases to separate and purify components. This technology is widely used in biochemistry, cell biology, biochemical industry, food industry, etc. due to its simple operation, low energy consumption, high separation efficiency, no damage and inactivation of the separated substances, easy engineering amplification and continuous operation, etc. Medicine and other fields, it is especiall...

Examples

experiment example 1

[0034] Experimental Example 1 The aqueous two-phase system based on anionic surfactants obtained in Example 1 was used to extract and purify biologically active substances. According to the total mass of the two-phase aqueous system, 1.0% (g / g) of sodium dodecylbenzene sulfonate and 39% (g / g) of 1-butyl-3 are weighed with a micro electronic balance. Methylimidazole tetrafluoroborate and 60.0% double distilled water, shake and stir evenly with a turbo agitator (Shanghai Jingke Instrument Co., Ltd.), use a micro syringe to inject 5-20μg of oil-soluble bell pepper pigment into the double water In the phase, shake, stir, and place at a constant temperature below 48°C. After 30 minutes, the solution separates. All capsicum pigments are extracted to the upper phase. This example proves that this system is an excellent extraction system for the separation and extraction of pepper pigments.

[0035] Experimental Example 2 The aqueous two-phase system formed based on anionic surfactants...

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