Method for preparing filial generation of dianthus caryophyllus and dianthus chinensis

Abstract

The invention discloses a method for preparing the filial generation of dianthus caryophyllus and dianthus chinensis, which comprises: 1, obtaining the protoplast of the dianthus caryophyllus and the dianthus chinensis respectively; 2, performing protoplast asymmetric fusion of the protoplast of the dianthus caryophyllus and the protoplast of the dianthus chinensis to obtain fused protoplast; and 3, culturing the fused protoplast to grow the protoplast into a hybrid plant. The invention provides the first method suitable for culturing the filial generation of the protoplast of the dianthus caryophyllus and the dianthus chinensis, overcomes the drawback that it is difficult to obtain the filial generation of the dianthus caryophyllus and the dianthus chinensis in the prior art and, by optimizing cross conditions, cultures novel filial generation which has good plant shape, high ornamental performance and high quantity of mu plant rate for the first time.

Description

technical field The invention belongs to the fields of botany and genetics, and more specifically, the invention relates to a method for preparing asymmetric hybrid protoplasts of carnation and Chinese carnation. Background technique Carnation (Dianthus caryophyllus) is one of the four major cut flowers in the world. It belongs to the genus Caryophyllaceae and is a perennial root herb. Plant height 30-60cm, stem clustered, smooth, slightly white powder, with enlarged nodes on the stem. Opposite leaves, linear-lanceolate, entire, base clasping, with white powder and grayish green, with 3-5 obvious veins. The flowers are mostly solitary at the top of the stem, and a few are clustered. The flower colors are white, pink, red, purple, yellow and variegated. They are fragrant and can be divided into single and double petals. Capsule, seeds black. Carnation is native to southern Europe and India. It was introduced to England in 1066, and there was a record of its cultivation in ...

AI Technical Summary

Problems solved by technology

However, due to the diversity of plants in nature, there are many types of cross-breeding methods in the prior art, and the cross-breeding methods that can be selected for different plants are also very different. The conditions are very difficult, and there is no report on the hybrid varieties of carnation and Chinese carnation in this field

Images