Common Anemarrhena asphodeloides extract with immunosuppressive activity and preparation method and medicinal purposes thereof

An Anemarrhena Anemarrhena extract and immunosuppressive technology are applied in pharmaceutical formulations, medical preparations containing active ingredients, drug combinations, etc., and can solve problems such as immunosuppressive activity that has not yet been seen

Active Publication Date: 2013-04-17
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern research has found that the chemical components in Anemarrhena are mainly steroidal saponins and bivapizones, as well as lignans, flavonoids, organic acids, etc. It is one of the hot drugs in modern medical research. In recent years The research progress of Anemarrhena is rapid, but most of them focus on timosaponins and bivapirox components. It is believed that timosaponin is an important medicinal material basis of Anemarrhena, and there are few studies on other components of Anemarrhena, including polysaccharides, and no other components have been found. Reported immunosuppressive activity

Method used

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  • Common Anemarrhena asphodeloides extract with immunosuppressive activity and preparation method and medicinal purposes thereof
  • Common Anemarrhena asphodeloides extract with immunosuppressive activity and preparation method and medicinal purposes thereof
  • Common Anemarrhena asphodeloides extract with immunosuppressive activity and preparation method and medicinal purposes thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take about 2.8 kg of Anemarrhena Anemarrhena, reflux extraction with 90% ethanol for 3 times, 2 hours each time, filter, dry the filter residue, add 10 times the amount of distilled water to the residue, reflux for extraction 3 times, 2 hours each time, filter, combine the filtrates, reduce The solvent was recovered by pressing to 1 volume of the medicinal material, adjusted to 80% alcohol content with 95% ethanol, left standing overnight, centrifuged at 2000 rpm / min, and the precipitate was washed with 4 times the volume of absolute ethanol and acetone in turn to obtain a crude extract. The crude extract was dissolved in water, the supernatant was put into a dialysis bag for dialysis under running water for 48 hours, distilled water was dialyzed for 24 hours, the dialysate was concentrated under reduced pressure to 1 volume of the medicinal material, 95% ethanol was added to adjust the alcohol content to 85%, left standing overnight, 2000 rpm / min centrifugation, the pr...

Embodiment 2

[0017] Take about 3kg of Anemarrhena Anemarrhena, reflux extraction with 80% ethanol for 2 times, 3h each time, filter, air dry the filter residue, add 8 times the amount of distilled water to the residue, reflux for extraction 2 times, 3h each time, filter, combine the filtrates, reduce pressure The solvent was recovered to 1 volume of the medicinal material, adjusted to 80% alcohol content with 95% ethanol, left standing overnight, centrifuged at 2000 rpm / min, and the precipitate was washed with 3 times the volume of absolute ethanol and acetone in turn to obtain a crude extract. The crude extract was dissolved in water, the supernatant was dialyzed under running water for 48 hours, distilled water was dialyzed for 24 hours, the dialysate was concentrated under reduced pressure to 1 volume of medicinal materials, 95% ethanol was added to adjust the alcohol content to 80%, left standing overnight, centrifuged at 2000 rpm / min, and precipitated. Washed with 3 times the weight of...

Embodiment 3

[0018] Embodiment 3 is made into infusion solution

[0019] The crude extract of Anemarrhena is further purified and separated through Sephacryl S300 and Sephadex G50 chromatography on agarose gel column: Anemarrhena polysaccharide extract with a purity of more than 98% is obtained, an appropriate amount of solubilizer is added, ground, and then a small amount of water for injection is added for dilution. Mix well, then add an appropriate amount of sodium chloride, dissolve and then add water for injection to the specified amount, filter, seal, and sterilize.

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PUM

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Abstract

The invention relates to a common anemarrhena polysaccharide extractive with immunosuppression activity and a preparation method and medicine purposes thereof. The preparation method comprising the steps of degreasing raw materials, extracting the degreased raw materials with water, precipitating the extracted material with alcohol, carrying out dialysis and ion-exchange column chromatography on the crude polysaccharide to eliminate proteins, pigments and molecular chemical components to produce an anemarrhena extractive. A huge amount of pharmacological experiments prove that common anemarrhena polysaccharide has an immunodepression activity and has the medical purposes of treating proinflammatory traumatic diseases, such as nephritis, pneumonia, upper respiratory tract infection, hepatitis, tonsillitis and diabetes.

Description

technical field [0001] The invention relates to an Anemarrhena Anemarrhena extract with immunosuppressive activity and a preparation method thereof, and the Anemarrhena Arneae extract is used as immunosuppressive activity for treating immune inflammatory injury diseases: nephritis, pneumonia, upper respiratory tract infection, Medicinal uses for hepatitis, tonsillitis and diabetes. Background technique [0002] Anemarrhena is the rhizome of Anemarrhena asphodeloides Bge., a plant of the Liliaceae Anemarrhena genus. It has the functions of clearing away heat and purging fire, nourishing yin and moisturizing dryness. It is used for exogenous fever, high fever and polydipsia, dry cough due to lung heat, hot flashes due to steaming of the bones, internal heat to reduce thirst, and intestinal dryness and constipation. Modern research has found that the chemical components in Anemarrhena are mainly steroidal saponins, diphenhydrazone, and there are lignans, flavonoids, organic ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8964C08B37/00A61P1/16A61P3/10A61P11/00A61P11/04A61P13/12A61P37/06
Inventor 匡海学王秋红王知斌夏永刚穆光锐薛娟
Owner 匡海学
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