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A kind of cinnamon bark polyphenol extract with immunosuppressive activity and its preparation method and application

An immunosuppression and extract technology, applied in the field of traditional Chinese medicine, can solve the problems of unclear material basis of drug efficacy and unclear mechanism of action.

Active Publication Date: 2018-04-13
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Li Yaohua and others reported (Sichuan Journal of Traditional Chinese Medicine, 1989, 2:28): 40 cases of rheumatoid arthritis were treated with 25g of cinnamon + 200g of mung bean + aged lime in an appropriate amount, 34 cases were markedly effective, and 6 cases were effective; but the mechanism of action is still unclear. The material basis for its medicinal effects has not yet been clarified.

Method used

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  • A kind of cinnamon bark polyphenol extract with immunosuppressive activity and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0022] a) Warm soaking and extracting the bark of Osmanthus osmanthus once at 40° C. with an aqueous acetone solution with a volume concentration of 50%, wherein: 1 gram of osmanthus bark uses 50 ml of aqueous acetone;

[0023] b) distilling off the acetone in the extract under reduced pressure, then adding an appropriate amount of water to dilute the concentrate to obtain a crude extract;

[0024] c) Extract the crude extract with ethyl acetate, the volume of ethyl acetate used in each extraction is 1 times the volume of the extracted crude extract, and extract 3 times in total; the ethyl acetate layers obtained by the combined extraction are distilled under reduced pressure Remove the ethyl acetate therein to obtain the ethyl acetate site;

[0025] d) Dissolve the obtained ethyl acetate part with a small amount of ethanol, add water to dilute and load the sample on the DM130 macroporous resin column, and use 4 column volumes of water, 4 column volumes of 10vol% ethanol aqueo...

Embodiment 2

[0028] a) At room temperature, use an acetone aqueous solution with a volume concentration of 50% to stir and extract the cassia osmanthus medicinal material twice, wherein: 1 gram of cassia osmanthus medicinal material uses 25 milliliters of aqueous acetone;

[0029] b) distilling off the acetone in the extract under reduced pressure, then adding an appropriate amount of water to dilute the concentrate to obtain a crude extract;

[0030] c) Extract the crude extract with ethyl acetate, the volume of ethyl acetate used in each extraction is 1 times the volume of the extracted crude extract, and extract 5 times in total; the ethyl acetate layers obtained by the combined extraction are distilled under reduced pressure Remove the ethyl acetate therein to obtain the ethyl acetate site;

[0031] d) Dissolve the obtained ethyl acetate part with a small amount of ethanol, add water to dilute and load the sample on the DM130 macroporous resin column, and use 4 column volumes of water,...

Embodiment 3

[0034] a) Carry out warm extraction twice at 40° C. with an acetone aqueous solution with a volume concentration of 20% of the cassia laurel bark medicinal material, wherein: 1 gram of the cassia osmanthus bark medicinal material uses 25 milliliters of acetone aqueous solution;

[0035] b) distilling off the acetone in the extract under reduced pressure, then adding an appropriate amount of water to dilute the concentrate to obtain a crude extract;

[0036] c) extract the crude extract with ethyl acetate, the volume of ethyl acetate used for each extraction is 1.5 times the volume of the extracted crude extract, and extract 2 times altogether; the ethyl acetate layers obtained by the combined extraction are distilled under reduced pressure Remove the ethyl acetate therein to obtain the ethyl acetate site;

[0037] d) Dissolve the obtained ethyl acetate part with a small amount of ethanol, add water to dilute and load the sample on the DM130 macroporous resin column, and use 4 ...

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Abstract

The invention discloses a cassia polyphenol extractive with immunosuppressive activity, and a preparation method and application thereof. The extractive is an extractive coming from at least one bark of cinnamomum japonicum, cinnamomum wilsonii and cinnamomum cassia, and the total polyphenol content in the extractive is 50 wt% or more. The half cell lethal concentration of the cassia polyphenol extractive is larger than 50 mu g / mL, has concentration-dependent inhibiting effect on propagation of lymphocyte induced by mitogen ConA and secretion of cytokines IL-6 and IFN-gamma important for immunoloregulation and inflammation reactions, is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating immunologic diseases, especially is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating rheumatoid arthritis, and has medicinal value.

Description

technical field [0001] The invention relates to a cinnamon bark polyphenol extract and its preparation method and application, in particular to a cinnamon bark polyphenol extract with immunosuppressive activity, its preparation method and application, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Rheumatoid arthritis (RA) is one of the most important diseases that endanger human health at present, and is called "the cancer that never dies". RA is a chronic systemic autoimmune inflammatory disease characterized by multi-joint involvement, synovial inflammation, and bone and cartilage destruction. Its pathogenesis has not yet been fully elucidated. disorders are closely related. The prevalence of RA in my country is about 0.3% to 0.4%, and there are about 4 million patients. The disability rate is 60% within 5-10 years of the disease course, and 90% of patients in the late stage lose social labor force and self-care ability,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/54A61P37/02A61P29/00A61P19/02
CPCA61K36/54A61K2236/333A61K2236/51A61K2236/55
Inventor 李医明杨以阜贾琦陈亮杨扬
Owner SHANGHAI UNIV OF T C M
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