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Neuroinvasion inhibitor

A nerve infiltration and inhibitor technology, which can be used in nervous system diseases, medical preparations containing active ingredients, antibodies, etc., and can solve problems such as lack of experience and unclear mechanism.

Inactive Publication Date: 2011-11-23
NAT CANCER CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of neural invasion and the symptoms caused by neural invasion are not clear, so the reality is that there is still a lack of experience in the control of neural invasion and the control of symptoms caused by neural invasion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] The intracellular mRNA expression of IL-6α receptor (IL6R) and IL-6β receptor (gp130) in human pancreatic cancer cell lines was investigated by real-time RT-PCR. In human pancreatic cancer cell lines, it was confirmed that IL6R mRNA ( figure 1 A) and gp130 mRNA ( figure 1 B) clear expression.

Embodiment 2

[0198] Using human recombinant IL-6, the cell number was measured over time ( figure 2 A and B), chemotaxis analysis ( figure 2 C), wound healing analysis ( figure 2 D) To study the effect of IL-6 on the proliferation ability, chemotaxis ability and migration ability of human pancreatic cancer cell lines. It was clarified that although IL-6 had no effect on the cell proliferation of pancreatic cancer cell lines, it enhanced the chemotaxis ability and migration ability.

Embodiment 3

[0200] Using recombinant human IL-6 (rhIL6), for phosphorylated STAT3 (pSTAT3) ( image 3 A), phosphorylated Erk1 / 2 (pErk1 / 2) ( image 3 B), phosphorylated Akt (pAkt) ( image 3 C), The effect of IL-6 on the intracellular signaling of human pancreatic cancer cell line Capan-1 was evaluated by Western blot. Fifteen minutes after adding rhIL6, the expression of phosphorylated STAT3 protein in the cells was significantly increased, and 1 hour after adding rhIL6, the expression of phosphorylated Erk1 / 2 protein was significantly increased. No effect on phosphorylated Akt expression was confirmed.

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Abstract

It is found that neuroinvasion in a neuroinvasion model for pancreatic cancer can be inhibited by inhibiting IL-6. Further, it is demonstrated that an IL-6 receptor is expressed in a human pancreatic cancer cell line and IL-6 can increase the chemotactic ability, the migratory ability and the intercellular signaling of a pancreatic cancer cell, and it is found that pancreatic cancer can be treated by inhibiting IL-6. Still further, it is found that human pancreatic cancer neuroinvasion can be inhibited by administering an IL-6 inhibitor to a mouse neuroinvasion model.

Description

technical field [0001] The present invention relates to inhibitors of neural invasion. More specifically, the present invention relates to an inhibitor of neural invasion comprising an interleukin 6 (IL-6) inhibitor as an active ingredient. Background technique [0002] Interleukin 6 (IL-6) is a cytokine also known as B cell stimulating factor 2 (BSF2) or interferon beta 2. IL-6 was discovered as a differentiation factor involved in the activation of B lymphocytes (Non-Patent Document 1), and later became clear that it is a multifunctional cytokine that affects the functions of various cells (Non-Patent Document 2). It has been reported that IL-6 induces the maturation of T lymphocyte-like cells (Non-Patent Document 3). [0003] IL-6 transmits its biological activity on cells through two proteins. One protein is the IL-6 receptor which is a ligand-binding protein with a molecular weight of about 80 kD to which IL-6 binds (Non-Patent Documents 4 and 5). IL-6 receptor exis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K39/395A61P1/18A61P25/00A61P35/00A61P43/00
CPCC07K16/2866A61K2039/505C07K2317/24C07K2317/76A61P1/18A61P25/00A61P35/00A61P43/00A61K39/001119
Inventor 光永修一落合淳志
Owner NAT CANCER CENT
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