Common lophatherum herb extract and preparation method and application thereof
A technology of light bamboo leaves and extracts, applied in the field of light bamboo leaf extracts and its preparation, can solve the problems of patents and literatures that have not found the use of flavonoid carbon glycosides in light bamboo leaves, and achieve good anti-respiratory virus activity and cytotoxicity small effect
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Embodiment 1
[0024] Grind the dry bamboo leaf medicinal material (purchased from the medicinal material market in Dongguan City, Guangdong Province, and identified by Professor Zhou Guangxiong, Jinan University Institute of Traditional Chinese Medicine and Natural Medicine), and extract it by percolation with 95% ethanol aqueous solution for 10 days, and filter the extract , the filtrate is concentrated into an extract with a rotary evaporator, and dried in a vacuum drying oven to form a brown powder, which is the crude extract of leaves of Pachyphyllum mellifera; the total flavonoids are 5-15% calculated by the weight of the dry product of the crude extract;
[0025] Weigh 5 kg of macroporous adsorption resin (D101), add 10 L of distilled water, stir evenly, remove resin monomer or fragments by water flotation, and then place it at room temperature for 24 hours; after the resin is fully swollen, drain the resin, and then use ethanol Wet column packing; then wash the column with ethanol unt...
Embodiment 2
[0028] Grind the dry bamboo leaf medicinal material, reflux extraction with 60% ethanol aqueous solution for 3 times, each time for 1 hour, filter the extract, combine the filtrate, concentrate the filtrate into an extract with a rotary evaporator, and store it in a vacuum drying oven Dried into a brown powder, which is the crude extract of Pachyphyllum edulis leaves; the crude extract contains 5-15% of total flavonoids based on the weight of the dry product;
[0029] The processing method of macroporous adsorption resin is consistent with embodiment 1. Take by weighing 200g of the dry product of the crude extract of Pachyphyllum edulis, dissolve it in 200mL of ethanol with a concentration of 95% by mass, and after ultrasonic degassing, add the sample to the processed macroporous resin column, and use 20L of ethanol with a concentration of 20% by mass first Elute the column with ethanol aqueous solution, and then use 50L mass percent concentration of 70% ethanol aqueous soluti...
Embodiment 3
[0030] Embodiment 3: the preparation of light bamboo leaf extract capsule
[0031] Take by weighing 50g of the obtained Pachyphyllum edulis extract in Example 1, add 10g of medicinal starch, mix evenly, granulate with 60mL of water or ethanol with a concentration of 95% by mass, pass through a 12-mesh sieve to extrude wet granules, and dry at 60°C Dry under vacuum, sieve with 20 meshes, add 5g micropowder silica gel at the same time, mix evenly, fill the particles into capsules, and obtain the capsules of the bamboo leaf extract.
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