Method for quickly identifying genetic purity of glutinous corn hybrid

An identification method, the technology of waxy corn, applied in the biological field, can solve the problems of low accuracy, heavy workload, and long time consumption, and achieve the effects of stable labeling, high accuracy, and improved efficiency
CN102505044BInactive Publication Date: 2013-08-07NANJING AGRICULTURAL UNIVERSITY
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2013-08-07
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of biology, and relates to a method for quickly identifying the genetic purity of a glutinous corn hybrid. The method comprises the following steps of: extracting genomic deoxyribonucleic acid (DNA) from glutinous corn, performing polymerase chain reaction (PCR) amplification by using the screened sequence-related amplified polymorphism (SRAP) effective primer combination NAUSRem7 / NAUSRpm1 and random amplified polymorphic DNA (RAPD) effective primers NAUSR709 and NAUSR712, respectively performing non-denaturing polyacrylamide gel electrophoresis and agarose gel electrophoresis on a PCR product obtained through amplification, and shooting a DNA electrophoresis pattern; and comparing and analyzing the size and position difference of polymorphism amplified fragments formed due to the difference of DNA fragment sequences in the electrophoresis pattern, and identifying the genetic purity of the glutinous corn F1 hybrid, namely a Suyunuo 2 seed. The detection method has the advantages of marking stability, high accuracy, no influence of the growth stage and environment of a sample to be detected, low cost, capability of being performed in thewhole growth season, and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for quickly identifying the genetic purity of waxy corn hybrids, which uses SRAP and RAPD molecular marker techniques to detect the genetic purity of seeds of the waxy corn variety 'Suyunuo 2'. Background technique

[0002] Seeds are extremely important means of production in agriculture, and the quality of seeds is directly related to the economic interests of seed producers, operators and users. The important basis for judging the quality of seeds is the four indicators of genetic purity, germination rate, clarity and moisture. The latter three index tests can be carried out indoors, but genetic purity identification is relatively difficult. Accurate, rapid and convenient identification of seed genetic purity is an important part of modern agricultural production and seed work. Maize belongs to Poaceae Zea (Zea) maize species (mays) (Gai Junyi. Monographs on Crop Breeding ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More