Endophytic fungi from podophyllum hexandrum plant and application of endophytic fungi

A fermentation product, Fusarium technology, applied in the field of endophytic fungi, can solve the problems of irreversible loss of biodiversity and ecological environment, difficult chemical total synthesis, complex chemical structure, etc., to protect plant ecological diversity, production cycle Short, easy-to-use effects

Inactive Publication Date: 2013-04-10
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increasing demand for podophyllotoxins in medicine, agriculture and forestry control, and the intensification of the collection of wild podophyllum plant raw materials, its resources have been severely damaged, resulting in irreversible losses of biodiversity and ecological environment ; Due to the complex chemical structure of podophyllotoxins, it is difficult to carry out chemical total synthesis; the construction of engineering bacteria by genetic engineering means is also impossible due to the large number of enzymes involved in the formation of such substances.

Method used

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  • Endophytic fungi from podophyllum hexandrum plant and application of endophytic fungi
  • Endophytic fungi from podophyllum hexandrum plant and application of endophytic fungi
  • Endophytic fungi from podophyllum hexandrum plant and application of endophytic fungi

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, isolation and identification of bacterial strain TG-9

[0027] 1. Separation

[0028] The fresh plant Sinopodophyllum hexandrum (Royle) Ying was collected from Xinglong Mountain (2300m above sea level) in Lanzhou City, Gansu Province. Roughly process the collected plant materials, select healthy and dry plant tissues, and wash the required parts (mainly roots or rhizomes and stems) with clean water to remove sediment and other impurities, and then wash them with filter paper. Remove residual moisture on the surface of plant tissue. In the ultra-clean workbench, treat with 75% ethanol for 1 min, rinse with sterile water for 5 times, then treat with 10% sodium hypochlorite for 6 min, then rinse with sterile water for 5 times, each time for 1 min, and finally rinse with 75% alcohol for 30 seconds, Rinse with sterile water 5 times, 1 min each time. Peel off the skin of the material with sterilized tweezers and blades, then cut into small pieces of 0.5cm×1cm...

Embodiment 2

[0038] Embodiment 2, bacterial strain TG-9 prepares podophyllotoxin

[0039] 1. Obtaining of fermentation broth

[0040] Put 100ml of prepared potato dextrose liquid culture medium in a 250ml Erlenmeyer flask, sterilize, inoculate Fusarium sp. (Fusarium sp.) TG-9CGMCC No.5528 mycelia (or spores) in the Erlenmeyer flask, at 28°C , 120r / min (radius of rotation: 2cm) shaker culture for 7 days to obtain the fermentation product.

[0041] Potato dextrose liquid culture medium is prepared according to the following method: Weigh 200g of potatoes (peeled, cut into about 2cm 2 small pieces), boil in water for 30 minutes, then filter with double gauze, take the filtrate and add 20g of glucose, then make up water to 1000mL, natural pH.

[0042] Two, the acquisition of the chloroform extract of fermented liquid

[0043] Use a suction filter to filter the fermentation product obtained in the above-mentioned step two times, collect mycelia and fermentation liquid (filtrate) respectively...

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Abstract

The invention discloses endophytic fungi from a podophyllum hexandrum plant and application of the endophytic fungi. The invention provides Fusarium sp. TG-9 with the collection number of GCMCC No.5528, and the application of the Fusarium sp. TG-9 with the collection number of GCMCC No.5528 in preparation of podophyllotoxin. Experiments prove that: the invention provides a bacterial strain which can be used for preparing the podophyllotoxin. A method for preparing the podophyllotoxin has the advantages of simple operation, low cost, and short production period. The invention provides a new way for preparing the podophyllotoxin, avoids the limitation of other preparation methods in the prior art, has high operability, is suitable for industrial large-scale production and has significance for protecting ecological diversity of plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an endophytic fungus derived from a plant called Pterocarpus chinensis and its application. Background technique [0002] Podophyllotoxin is an important aryl tetralin lignan, a natural compound with a wide range of biological activities, including estrogen-like, anti-estrogenic, anti-cancer, anti-viral, anti-immune, antibacterial Insects, treatment of psoriasis, malaria, rheumatoid arthritis, multiple sclerosis, inhibition of plant growth, anti-oxidation, etc. It is the precursor of the synthesis of anticancer drugs VP-16 (etoposide) and VM-26 (teniposide) in recent years substance. VP-16 and VM-26 have good curative effects on various tumor diseases such as small cell lung cancer and lymphoma, and have been developed as anticancer drugs for clinical application. [0003] At present, podophyllotoxin is mainly extracted from the rhizomes of wild podophyllum plants, and there are on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/18C12R1/77
Inventor 赵长琦朱妍妍梁子臻张嘉钱沚莹
Owner BEIJING NORMAL UNIVERSITY
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