Method for preparing podophyllotoxin and/or astragaloside and special bacterial strain thereof

A technology for podophyllotoxin and astragaloside, applied in biochemical equipment and methods, microorganism-based methods, fungi, etc., can solve the problems of difficult large-scale artificial cultivation, difficult simulation, long growth cycle, etc., and achieve protection The effect of plant ecological diversity, strong operability, and short production cycle

Inactive Publication Date: 2011-09-21
BEIJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complex growth environment of plants, it is difficult to simulate, and its growth cycle is long, it is difficult to carry out large-scale artificial cultivation, and tissue culture technology is not suitable for podophyllum plants; in addition, the chemical structure of podophyllotoxins is complex, chemical synthesis Although it can be carried out, the cost is too high, and the construction of engineered bacteria by means of genetic engineering cannot be carried out due to the large number of enzymes involved in the formation of such substances.

Method used

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  • Method for preparing podophyllotoxin and/or astragaloside and special bacterial strain thereof
  • Method for preparing podophyllotoxin and/or astragaloside and special bacterial strain thereof
  • Method for preparing podophyllotoxin and/or astragaloside and special bacterial strain thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1, separation and identification of Penicillium ateckii (Penicillium ateckii) WEQE CGMCC №3401

[0029] 1. Separation

[0030] The roots and rhizomes of the plant Diphyllria sinensis L. collected from the Red River Valley (1560m above sea level) in Meixian County, Shaanxi Province were washed with tap water, and treated with 75% ethanol by volume in an ultra-clean workbench. 5 minutes, then rinse with sterile water for 3-5 times; then treat with 2.5% by mass sodium hypochlorite for 10 minutes, and then rinse with sterile water for 3-5 times. Use sterilized tweezers and razor blades to peel off the outer skin of the roots of Woerqi, cut them into small pieces of 0.5cm×0.5cm, plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days. At the same time, the roots of Woerqi treated with the same sterilization were not peeled and cut, and the roots of Woerqi were rolled on the PDA medium for one week, and cultivated under the same conditions as a con...

Embodiment 2

[0042]Example 2, Utilizing Penicillium ateckii (Penicillium ateckii) WEQE CGMCC №3401 to prepare podophyllotoxin and astragaloside

[0043] 1. Culture strains

[0044] Put 100ml of prepared potato dextrose liquid culture medium in a 250ml Erlenmeyer flask, sterilize, pick a single colony of Penicillium ateckii (Penicillium ateckii) WEQE CGMCC №3401 and inoculate it in the above sterilized potato dextrose liquid medium , 28° C., 120 r / min shaker culture for 7 days to obtain a fermentation broth.

[0045] Potato dextrose liquid culture medium is prepared according to the following method: Weigh 200g of potatoes (peeled, cut into about 2cm 2 small pieces), boil in water for 30 minutes, then filter with double gauze, take the filtrate and add 20g of glucose, then make up water to 1000mL, natural pH.

[0046] The experiment was repeated 3 times.

[0047] Second, the extraction of the product

[0048] The fermentation broth was filtered twice with a suction filter, and the mycel...

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Abstract

The invention discloses a method for preparing podophyllotoxin and / or astragaloside and a special bacterial strain thereof. The bacterial strain is Penicillium ateckii WEQE of which the collection number is CGMCC NO.3401. The bacterial strain in the invention can be used for preparing podophyllotoxin and / or astragaloside. The method for preparing podophyllotoxin and / or astragaloside in the invention is not influenced by plant resources, has the advantages of simple operation, low cost, easy operation, short production cycle and the like, provides a new path for preparing podophyllotoxin, avoids limitations of other preparation methods in the prior art, has strong maneuverability, is suitable for large-scale industrialized production, and has important significance on protecting ecological diversity of plants.

Description

technical field [0001] The invention relates to a method for preparing podophyllotoxin and / or astragaloside and special strains thereof. Background technique [0002] Podophyllotoxin (podophyllotoxin) mainly exists in the Berberidaceae Podophyllum and its related plants. It is a class of natural compounds with a wide range of biological activities, including estrogen-like, anti-estrogen-like, anti-cancer, anti-viral , antifungal, sedative, inhibiting plant growth and antioxidant effects. In particular, it has strong anticancer activity, and its mechanism of action is to inhibit the depolymerization of tubulin to prevent cell division and inhibit the activity of DNA topoisomerase. The glycoside derivatives of podophyllotoxin, etoposide and teniposide, have low toxicity and have good curative effects on various tumor diseases such as small cell lung cancer and lymphoma, so they have become broad-spectrum anti-tumor drugs for clinical application. [0003] At present, podophy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/18C12P19/60C12R1/80
Inventor 赵长琦朱妍妍艾嫦
Owner BEIJING NORMAL UNIVERSITY
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