Linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof

A poultry reticuloendothelial and epitope polypeptide technology, applied in the direction of application, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of complex operation and high cost

Inactive Publication Date: 2013-11-13
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional antigen epitope analysis method, that is, antibody screening overlapping synthetic peptide method, needs to clarify the amino acid sequence of the antigen in advance, and then synthesize a large number of peptides for screening. The cost is high and the operation is relatively complicated. For glycosylated antigens and conformational antigens The limitations of bit screening are more obvious

Method used

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  • Linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof
  • Linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof
  • Linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof

Examples

Experimental program
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Embodiment 1

[0019] Example 1 Screening of epitopes

[0020] 1. Purification of monoclonal antibodies

[0021] Utilize the Recombinant Protein G Agarose (rProtein G Agarose) of Invitrogen Company to purify the monoclonal antibody, the specific steps are as follows:

[0022] (1) Add 1ml rProtein G Agarose to the adsorption column;

[0023] (2) Add 10ml binding buffer to equilibrate the adsorption column;

[0024] (3) After all flow is exhausted, cover the bottom of the adsorption column with a cap, add 700 μl of monoclonal antibody sample to the adsorption column, let it react at room temperature for 30 minutes, and shake it from time to time to ensure that the gel filler is always in a suspended state;

[0025] (4) Move the adsorption column into a new collection tube, open the bottom cover, and let all the monoclonal antibodies flow down;

[0026] (5) Add 6-12ml binding buffer to the adsorption column to wash away unbound protein;

[0027] (6) Move the adsorption column into a new col...

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Abstract

The invention discloses a linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof. According to the invention, a random 12-peptide library (Ph.D.-12TM) of M13 bacteriophage is used for performing biological selection on monoclonal antibody A9E8 with neutralizing activity of the fowl reticuloendotheliosis gp90 protein for three times, so as to obtain 8 bacteriophage-positive clones, which can perform specific combination reaction with A9E8; and then fusion 12 peptides of the positive clones are subjected to sequencing and sequence comparison to analyze that the epitope peptide motif identified by A9E8 is SVQYHPL, and sequence comparison discovers that the motif is highly conserved in the fowl reticuloendotheliosis virus group, so that the epitope is inferred to be the fowl reticuloendotheliosis virus group specific epitope. According to the invention, theoretical basis is provided for development of gene engineering diagnosis reagents and design of polypeptide vaccines based on epitope.

Description

technical field [0001] The invention relates to an antigenic epitope polypeptide and its application, in particular to a neutralizing antigenic epitope polypeptide for gp90 protein of avian reticuloendothelial hyperplasia. It belongs to the field of molecular detection. Background technique [0002] Avian reticuloendotheliosis is a group of turkeys, chickens, ducks, crane quails and geese caused by avian reticuloendotheliosis virus (Retieuloendotheliosis Viruse, REV) group characterized by lympho-reticular cell proliferation Syndrome (Witter RL, Fadly A M. Reticuloendoetheliosis. In Disease of Poultry, 11th edn, 2003, 517-535. Edited by Y M Saif, H J Barnes, J R Glisson, et al. Ames, IA, USA: Iowa State Press. ), these syndromes include acute reticulocyte neoplasia, growth arrest syndrome, and chronic neoplasia of lymphoid and other tissues. The current REV population includes defective T strains, chick syncytial virus, duck infectious anemia virus, spleen necrosis virus, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/40G01N33/68G01N33/569C12Q1/70C12Q1/68C12R1/93
Inventor 王云峰赵妍石星明崔红玉王玫
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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