Optical projection tomography method based on merged spiral scanning mode

Abstract

The invention discloses an optical projection tomography method based on a merged spiral scanning mode. The optical projection tomography method comprises the following steps of: merging projection drawings on the same height, at the same angle and at different detector positions into a projection drawing which can cover all samples in the horizontal direction, and merging a plurality of local spiral projection data into a whole spiral projection data; calculating the axial visual field of the whole three-dimensional reconstruction body according to the axial position and scanning angle of each merged projection drawing; dividing the three-dimensional reconstruction body into a plurality of pieces of axial tomography to be reconstructed, performing data rearrangement on a projection line corresponding to each axial tomography to be reconstructed to obtain a sinusoidal chart corresponding to the axial tomography to be reconstructed; and reconstructing the sinusoidal chart into a tomography image by utilizing a circular track filtering back projection method, and sequentially superposing various tomography images to obtain the three-dimensional reconstruction body. According to the method, the visual filed of the optical projection tomography can be effectively widened, and the capacity of imaging a large-size object in an imaging system is improved.

Description

technical field [0001] The present invention relates to an optical projection tomography (Optical Projection Tomography, OPT) technology, in particular to an optical projection tomography method based on a flattened helical scan mode. Background technique [0002] Optical projection tomography technology uses the characteristic that light propagates along a straight line in a small-sized organism, and uses a camera to collect images from multiple angles for three-dimensional imaging. At present, OPT is divided into transmission type and excitation type. The transmission type passes light directly through the sample, and uses the absorption characteristics of the sample to obtain the three-dimensional anatomical structure image of the sample; the excitation type irradiates the sample with laser light to excite the fluorescent dye or Fluorescent proteins emit fluorescent signals with longer wavelengths, reconstructing the distribution information of fluorescent dyes or fluores...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that optical projection tomography circular orbit scanning cannot finely image large-sized objects, the present invention provides an optical projection tomography method based on a flattened spiral scanning method, by performing multiple partial spiral scans on the sample to realize the image of the sample Three-dimensional imaging, improving the imaging field of view, accuracy and speed of optical projection tomography for large-scale objects

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