Multi-parameter electrochemical immunosensor based on electrode array and preparation method thereof

Abstract

The invention discloses a multi-parameter electrochemical immunosensor based on an electrode array and a preparation method thereof, relating to the biosensor technology and the bioelectronics technology. The sensor is integrated with the electrode array, a counter electrode, an electrochemical reference electrode, a lead and an interface on a substrate. According to the sensor, the micro electro mechanical system (MEMS) technology is adopted to process and prepare the basic electrode array, silver and silver chloride (Ag / AgCl) are used for modifying the counter electrode and the electrochemical reference electrode, and electric media bodies, porous shielding films, nano materials, antibodies and the like are fixed on a detection electrode to form a multi-parameter detection system. The sensor has the characteristics of good stability and convenience for use, and can simultaneously detect a plurality of lung cancer biomarkers, so as to facilitate clinical application.

Description

technical field [0001] The invention relates to biosensors and bioelectronics, in particular to a multi-parameter electrochemical immunosensor based on an electrode array and a preparation method thereof. Background technique [0002] At present, lung cancer is the cancer with the highest incidence rate in the world, with 1.2 million new patients every year; the total number of lung cancer patients in my country ranks first in the world. In 2005, the data of our country's assessment of lung cancer showed that there were 500,000 new patients. Tumor markers are chemical substances that reflect the presence of tumors. They either do not exist in normal adult tissues but are only found in embryonic tissues, or their content in tumor tissues greatly exceeds that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the histogenesis and cell differentiation of tumors , cell function, to help tumor diagnosis, classificati...

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Problems solved by technology

However, at present, large-scale instruments are used for the detection of these markers, and samples need to be sent to special laboratories, so rapid on-site detection cannot be achieved.

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