Flight mass spectrum biochip for innate potential assessment and its detection method

A technology of flight mass spectrometry and biochip, which is applied in the field of flight mass spectrometry biochip and its detection for talent potential assessment, which can solve the problems of lack of advance career planning in life and the inability to effectively evaluate personal talent potential

Inactive Publication Date: 2013-04-24
上海华翼生物科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems in the prior art that the individual's talent potential cannot be effectively evaluated, and there is no advan

Method used

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  • Flight mass spectrum biochip for innate potential assessment and its detection method
  • Flight mass spectrum biochip for innate potential assessment and its detection method
  • Flight mass spectrum biochip for innate potential assessment and its detection method

Examples

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Embodiment Construction

[0011] The experimental method that does not indicate specific condition in the following examples, usually according to conventional conditions, such as Sambrook et al., molecular cloning: the conditions described in the laboratory manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the manufacturer suggested conditions.

[0012] Step 1 Extract DNA from the subject sample: use Wizard Genomic DNA purification Kit (Promega) or Tissue (MN) extracts DNA from tissues, cell blood samples or oral mucosa, quantifies it with a spectrophotometer, and checks its quality by agarose gel electrophoresis. The concentration of qualified DNA was adjusted to 50ηg / l, and stored at -20°C for future use.

[0013] Step 2 Design PCR primers and single-base extension primers: design PCR primers and single-base extension primers for SNP sites (see Table 1).

[0014] Table 1

[0015]

[0016]

[0017]

[0018] Step 3 Perform PCR amplification of the target gene o...

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PUM

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Abstract

The invention discloses a flight mass spectrum biochip for innate potential assessment. The biochip includes a sample analyte and a mass spectrum chip matrix that undergo cocrystallization. The sample analyte comprises a target gene, a PCR primer and a single base extension primer used for innate potential assessment. The invention also discloses a detection method through the chip. The method consists of: extracting the sample DNA of a detection subject; designing a PCR primer and a single base extension primer; conducting target gene PCR amplification and single base extension on the sample DNA to form a sample analyte; and subjecting the sample analyte and the mass spectrum chip matrix to cocrystallization, and detecting the SNP locus of the target gene. The gene chip provided in the invention can get an in-depth understanding of the potential ability of a person, make assessment on individual innate potential, and forecast the problems a person may encounter in the growth process in advance, thus avoiding receiving education blindly. Therefore, the biochip provided in the invention can help the detection subject to make targeted life career planning.

Description

technical field [0001] The invention relates to a gene chip, in particular to a flight mass spectrometry biochip for evaluating talent potential and a detection method thereof. Background technique [0002] The existing technology lacks a gene chip that can detect the potential of an individual's talent, so as to deeply understand the potential ability of the individual, predict the problems that the individual may encounter during growth, including psychological problems, and avoid blindly cultivating children. Targeted life and career planning for children. Contents of the invention [0003] In order to overcome the problems in the prior art that the individual's natural potential cannot be effectively evaluated and the lack of advance career planning in life, the present invention provides a flight mass spectrometry biochip for evaluating natural potential and a detection method thereof. [0004] A flying mass spectrometry biochip for evaluating innate potential, compr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/62G01N27/626
Inventor 张成虎
Owner 上海华翼生物科技有限公司
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