Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for obtaining polyclonal antibody of sheep independent growth factor 1B

A polyclonal antibody, growth factor technology, applied in the direction of anti-growth factor immunoglobulin, anti-animal/human immunoglobulin, serum immunoglobulin, etc., can solve problems such as no sheep

Inactive Publication Date: 2013-05-08
GUIZHOU UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the GFI1B gene sequence of humans, cattle, mice and other animals has been searched in the GenBank data, but the GFI1B gene sequence of sheep has not been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for obtaining polyclonal antibody of sheep independent growth factor 1B
  • Method for obtaining polyclonal antibody of sheep independent growth factor 1B
  • Method for obtaining polyclonal antibody of sheep independent growth factor 1B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0089] Embodiment of the present invention: the acquisition method of the polyclonal antibody of sheep independent growth factor 1B, using the designed specific primer GFI-1 to PCR amplify the sheep independent growth factor 1B gene to obtain its CDS sequence; and through the specificity Primer GFI-2 was used for enzyme digestion, and the prokaryotic expression vector was constructed to obtain the prokaryotic expression vector pET32a-GFI1B; then the prokaryotic expression vector pET32a-GFI1B was induced to express the target gene, and the sheep independent growth factor 1B protein was obtained; the sheep independent growth factor 1B protein was purified to more than 85%; the purified sheep-independent growth factor 1B protein was immunized on animals, and whole blood was collected and serum was separated one week after the last immunization; after the serum was purified, the target sheep-independent growth factor 1B protein was obtained Polyclonal antibody; specific primer GFI-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for obtaining a polyclonal antibody of a sheep independent growth factor 1B, which comprises the following steps: performing PCR (polymerase chain reaction) amplification on a sheep independent growth factor 1B gene by using a designed specific primer GFI-1, thus obtaining a CDS sequence thereof; performing enzyme cutting by using a specific primer GFI-2, and constructing a prokaryotic expression vector, thus obtaining the prokaryotic expression vector pET32a-GFI1B; performing target gene induced expression on the prokaryotic expression vector pET32a-GFI1B, thus obtaining a sheep independent growth factor 1B protein; purifying the sheep independent growth factor 1B protein to 85% or above; immunizing the purified sheep independent growth factor 1B protein onto an animal, collecting whole blood one week after the last immunization, and separating the blood serum; and purifying the blood serum, thus obtaining the polyclonal antibody of the target sheep independent growth factor 1B gene.

Description

technical field [0001] The invention relates to a biological technology, in particular to a method for obtaining a polyclonal antibody of goat independent growth factor 1B. Background technique [0002] Growth factor independent 1B GFI1B (growth factor independent 1B GFI1B) is the second gene discovered in the GFI1 family. In 1933, Gilks ​​et al. first discovered the GFI1 gene at the MOMULV integration site of rat T-cell lymphoma. It is located on chromosome 1p22 of the human genome. Rodel et al. discovered the GFI1B gene in the mouse and human genomes in 1998, located on chromosome 9q34.13 of the human genome. Studies have shown that it is a nuclear transcriptional repressor, encoding 329 amino acids, which contains an N-terminal SNAG region consisting of 20 amino acids and a C2H2-like zinc finger region with 6 carboxy-terminals. The part between the two regions of GF11B consists of 144 amino acids. FI1B is mainly expressed in fetal and adult erythroid and megakaryocyte l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/22C07K16/06
Inventor 陈志罗卫星刘若余石照应陈颖杨海兵李世功龙国荣宋桃伟杨永强蔡惠芬张依裕李维静
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com