Construction method of plant dual-gene co-expression vector
A technology of co-expression vector and construction method, which is applied in the field of construction of plant dual-gene co-expression vector, can solve the problems of affecting the efficiency of metabolic pathways, and the expression does not tend to be balanced, and achieves the effect of simplifying the screening procedure.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0028] When implementing the present invention, take the construction of the co-expression vector of HbWRKY11 and HbWRKY7 as an example for further description, and the specific steps are as follows:
[0029] 1. Design primers
[0030] 1) Design primers, SP-F and SP-R, according to the spacer sequence to be amplified, and introduce Ncol and EcoR Ⅰ restriction sites into the upstream primer and downstream primer, respectively;
[0031] 2) According to the structural characteristics of the multiple cloning site of the pXCS-HAStrep vector, design linkers SP-A1 and SP-A2.
[0032] 2. Vector construction
[0033] 1) Using the vector pGWB605 (GenBank accession number AB543114) as a template and SP-F / SP-R as primers, the spacer sequence was obtained by PCR amplification, and the product size was 341bp;
[0034] 2) Ligate the amplified prod...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com