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Identification method of oligofructose inulin purity

A technology of fructooligosaccharides and identification methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve complex problems such as inability to correctly detect product purity, etc.

Active Publication Date: 2014-04-16
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With high performance liquid chromatography, the method of measuring inulin content with a certain monomer component of inulin polysaccharide as a standard is more complicated, because the monomer component of domestic inulin is not easy to obtain, so the current routine analysis method sulfuric acid-phenol method The purity of the product cannot be detected correctly

Method used

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  • Identification method of oligofructose inulin purity
  • Identification method of oligofructose inulin purity
  • Identification method of oligofructose inulin purity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0023] 1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5mL volumetric flask, prepare a fructose standard solution with a concentration of 10mg / mL, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance In a 10mg to 10mL volumetric flask, prepare a glucose standard solution with a concentration of 1mg / mL, and pass through 0.45um to be tested;

[0024] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: Phenomenex Rezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50mL / min; Injection volume: 25uL; Run time: 30min:

[0025] 3) Inulin sample processing: Test product (1): Accurately weigh 1.0 g of inulin sample and put it in a 100 mL volumetric flask, add an appropriate amount of water, heat to dissolve, cool to ...

Embodiment 2

[0029] 1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5mL volumetric flask, prepare a fructose standard solution with a concentration of 10mg / mL, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance In a 10mg to 10mL volumetric flask, prepare a glucose standard solution with a concentration of 1mg / mL, and pass through 0.45um to be tested;

[0030] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: PhenomenexRezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50mL / min; Injection volume: 25uL; Run time: 35min:

[0031] 3) Inulin sample processing: Test product (1): Accurately weigh 1.0 g of inulin sample, put it in a 100 mL volumetric flask, add an appropriate amount of water, heat to dissolve, cool to a gr...

Embodiment 3

[0035]1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5mL volumetric flask, prepare a fructose standard solution with a concentration of 10mg / mL, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance In a 10mg to 10mL volumetric flask, prepare a glucose standard solution with a concentration of 1mg / mL, and pass through 0.45um to be tested;

[0036] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: Phenomenex Rezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50mL / min; Injection volume: 25uL; Running time: 35min; Running time: 40min;

[0037] 3) Inulin sample processing: Test product (1): Accurately weigh 1.0 g of inulin sample and put it in a 100 mL volumetric flask, add an appropriate amount of water, he...

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Abstract

The invention discloses an identification method of an oligofructose inulin purity. The method comprises the following steps of: carrying out enzymatic hydrolysis on inulin by using inulase, then determining contents of fructose and glucose in a sample before the enzymatic hydrolysis and after the enzymatic hydrolysis of oligofructose inulin by using HPLC(High Performance Liquid Chromatography), respectively calculating content differences of fructose and glucose before the enzymatic hydrolysis and after the enzymatic hydrolysis, and multiplying the sum of the percentage difference before the enzymatic hydrolysis and after the enzymatic hydrolysis of fructose and the percentage difference before the enzymatic hydrolysis and after the enzymatic hydrolysis of glucose by 0.9 to be converted into the purify of inulin. The identification method particularly comprises the following steps of: 1) preparing a fructose and glucose reference solution; 2) setting chromatographic conditions; 3) treating an inulin sample; 4) analyzing and calculating the contents of fructose and glucose by using the HPLC method; and 5) identifying the inulin purity. Through the identification method of the oligofructose inulin purity disclosed by the invention, enzymatic hydrolysis is carried out on inulin by using inulase, then the HPLC is used to analyze changes of fructose and glucose before the enzymatic hydrolysis and after the enzymatic hydrolysis of oligofructose inulin, and finally the inulin purity is accurately identified, thereby judging whether a product is qualified or not. In comparison with the traditional method, the identification method is more scientific and reasonable.

Description

technical field [0001] The invention relates to a method for determining the difference in fructose and glucose content between inulin before enzymolysis and after enzymolysis by high-performance liquid chromatography, and calculating the true purity of the inulin. Background technique [0002] Inulin belongs to the linear inulin-type fructan. As a natural functional polysaccharide, it has the physiological functions of water-soluble dietary fiber and biological activity prerequisites, and has a super-strong function of proliferating bifidobacteria. Balance the body, restore gastrointestinal function, improve immunity, promote the absorption of mineral calcium, magnesium, zinc, copper, etc., regulate blood sugar, lower blood lipids, prevent obesity and anti-cancer and other physiological functions, prevent various diseases, and maintain good health It plays an extremely important role and can be widely used in low-calorie, low-sugar, and low-fat foods. According to literatu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 高平陈华李荣张国栋张沛霞
Owner JIANGSU ALAND NOURISHMENT
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