Application and construction method of animal model of insulin resistance induced by high selenium

An insulin resistance, animal model technology, applied in medical preparations containing active ingredients, pharmaceutical formulations, sulfur/selenium/tellurium active ingredients, etc. Overcome the limitations of research and related drug development, reduced levels of protein kinase B phosphorylation, and reproducible effects

Inactive Publication Date: 2015-09-16
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the narrow therapeutic window of selenium, excessive levels of selenium may also cause negative effects on the human body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application and construction method of animal model of insulin resistance induced by high selenium
  • Application and construction method of animal model of insulin resistance induced by high selenium
  • Application and construction method of animal model of insulin resistance induced by high selenium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0028] The present invention is administered according to a dosage of 100 μg / kg-200 μg / kg every day to induce; high selenium induces glucose and lipid metabolism disorder, and then induces the occurrence of insulin resistance. The animal model of insulin resistance constructed by this method has characteristics similar to those of human high selenium insulin resistance.

[0029] The measurement results of the constructed animal model of insulin resistance showed that: the glucose tolerance and insulin tolerance of the animals in the model group were significantly different from those in the normal control group, the phosphorylation level of protein kinase B stimulated by insulin in the animals in the model group was significantly reduced, and the key to gluconeogenesis in the model group was ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application and construction method of a high-selenium induced insulin resistance animal model. The application and construction method comprises the following steps of: feeding 100mug / kg-200ug / kg of selenium to the stomach of an SD (Sprague Dawley) rat every day; and six weeks later, obtaining the insulin resistance animal model. According to the statistic analysis of glucose tolerance and insulin tolerance, insulin signal transduction and gluconeogenesis key enzyme test, the construction of the insulin resistance animal model by utilizing sodium selenite is feasible; and the insulin resistance animal model can be used as a high-selenium induced insulin resistance animal model and a pathogenesis and drug action mechanism research model of diabetes mellitus II. The insulin resistance rat model constructed by the application and the construction method disclosed by the invention has the advantages of simpleness in modeling method, high model establishing success rate, low cost, short period, good reproducibility and the like.

Description

technical field [0001] The invention belongs to the technical field of diabetes prevention and treatment, and relates to the application and construction method of an animal model of insulin resistance induced by high selenium. Background technique [0002] The construction of animal models is a very important technology in medical research. Constructing a disease model similar to human disease can overcome the constraints of long incubation period, many influencing factors, slow development and complex pathogenesis of human disease, better reflect the law of disease occurrence and development, and facilitate the study of different diseases. Diseases that are easy to study in humans themselves. Commonly used animal models mainly include spontaneous animal models, transgenic animal models and experimental animal models. Spontaneous animal models and transgenic animal models have high application value, but the high cost of modeling and high requirements for technical breedi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K33/04
Inventor 王欣海春旭
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap