Detection method for CVA10 in environmental water body

A detection method and virus technology, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc., which can solve the problem of indistinguishable identification by cell culture method, low CVA10 concentration, and inability to accurately qualitatively or quantitatively detect CVA10, etc. question
CN103305630BInactive Publication Date: 2015-07-01XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
Publication Date
2015-07-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection method for CVA10 in an environmental water body, wherein a semi-nested RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and real-time fluorescence quantification RT-PCR reaction system is established to detect CVA10 virus in the environmental water body by using a recombinant plasmid as a reference product, and the recombinant plasmid is prepared by using a primer and a probe aiming at CVA10 virus designed according to a genetic conservative fragment of enterovirus belonging to a VP1 serotype area. The method has good specificity and high sensitivity and precision, and can qualitatively and quantitatively detect CVA10 virus in the environmental water body accurately.
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Description

technical field

[0001] The invention belongs to the technical field of detection of environmental water quality, and in particular relates to a detection method of CVA10 virus in environmental water. technical background

[0002] Coxsackievirus A10 (CVA10) is one of the main pathogens causing HFMD and belongs to the Enterovirus genus. CVA10 is a water-borne virus that can be released into environmental waters from the secretions of CVA10-infected patients and can survive in environmental waters for a long time. The water body polluted by it becomes an important pollution source of CVA10, which can cause the outbreak of hand, foot and mouth disease.

[0003] Existing methods for detecting CVA10 mainly include cell culture method and polymerase chain reaction (Polymerase Chain Reaction, PCR). Because there are a variety of pathogenic microorganisms in the environmental water, especially other enteroviruses, these microorganisms interfere with the detection of CVA10 by cell c...

Claims

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