Cell printing method and cell printing system

Abstract

The invention discloses a cell printing method and a cell printing system. The cell printing method comprises the steps that S1, a microjet tube is inserted into a required cell suspension; S2, predetermined cell suction driving is executed on the microjet tube by utilizing a microdisplacement reciprocating mechanism; a certain number of cells are sucked; a cell printing sequence is combined; S3, a predetermined cell gathering driving is executed on the microjet tube by utilizing the microdisplacement reciprocating mechanism; the cells are arrayed into a close-packed single cell row in the microjet tube; and S4, the microjet tube is moved to a required printing position; a predetermined cell jet driving is executed on the microjet tube by utilizing the microdisplacement reciprocating mechanism; and the cells are jetted at the printing position according to the sequence. The cell printing method can utilize the same microjet tube to realize suction and printing operation of multiple cells, so that equipment complexity increase by adopting multiple microjet tubes is avoided, and operation process complication due to replacement of the microjet tubes is avoided.

Description

technical field [0001] The invention relates to the fields of cell printing and cell extraction, in particular to a cell printing method and a cell printing system. Background technique [0002] In recent years, cell printing technology has played an increasingly important role in the fields of tissue engineering, pathological model construction, drug screening and detection, and cell biology research. At present, cell printing technologies mainly include inkjet printing (piezoelectric volume-driven and thermal bubble), laser direct writing, laser-induced transfer, electrostatic spraying, focused ultrasonic jetting, micro-extrusion, etc. The above-mentioned cell printing technologies exist as follows Problems: 1. In the process of cell printing, instantaneous high temperature, high voltage or instantaneous strong electrostatic field are often generated, which are not good for the survival of cells or maintaining their original biological characteristics. 2. When spraying va...

AI Technical Summary

Problems solved by technology

At present, cell printing technologies mainly include inkjet printing (piezoelectric volume-driven and thermal bubble), laser direct writing, laser-induced transfer, electrostatic spraying, focused ultrasonic jetting, micro-extrusion, etc. The above-mentioned cell printing technologies exist as follows Problems: 1. In the process of cell printing, instantaneous high temperature, high voltage or instantaneous strong electrostatic field are often generated, which is not conducive to the survival of cells or the maintenance of their original biological characteristics.

2. When spraying various types of cells, it is often necessary to use multiple sets of spraying mechanisms or replace multiple spray heads, which increases the complexity of the equipment or increases the complexity of the operation.

3. The minimum number of cells printed by most processes is still in the range of several to dozens, and the stable and efficient single-cell printing has not been fully developed

Images