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Cell proliferation bag and cell proliferation device

A technology of cell proliferation and bag body, which is applied in the field of cell proliferation, can solve the problems of cell proliferation that cannot be actively supplied with oxygen, difficulty in meeting the needs of cell proliferation, and lack of gas path connection channels, etc., to save manpower, increase pressure, and improve Effect of dissolved oxygen level

Active Publication Date: 2013-10-23
GUANGZHOU ZHONGXI MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The passive method of natural infiltration is difficult to meet the needs of cell proliferation under high-density conditions
In the past, the cell proliferation bag did not have an air connection path, and could not use active oxygen supply to achieve cell proliferation
[0006] In the past, the cell proliferation bag used the liquid flow path on the side of the bag. In the actual culture state, these paths are directly in contact with the liquid below the liquid level, and the gas path cannot be connected.

Method used

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  • Cell proliferation bag and cell proliferation device
  • Cell proliferation bag and cell proliferation device
  • Cell proliferation bag and cell proliferation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A cell proliferation bag such as figure 1 As shown in -3, it includes a bag body 1 with sealed edges; the bag body 1 is provided with a guide body 2, the guide body is attached to the bottom surface of the bag body, and the bottom surface of the guide body has a diameter of 8cm and a height of 5cm.

[0043] The bottom surface of the conducting body is circular, and its vertical cross section is triangular, that is, the conducting body is a cone.

[0044] There are three said conducting bodies, which are evenly distributed on the bottom side of the bag body, and are placed in a staggered position at the three-point line of the bottom of the bag. The bag body is made of a soft film material with ultra-high gas barrier; such as Figure 4 As shown in -8, the top surface of the bag body is provided with a first adapter pipe 3 that communicates the inside and the outside of the bag body. There are two first adapter pipes 3, and one end of the first adapter pipe 3 that commun...

Embodiment 2

[0051] The cell proliferation bag in this embodiment is basically the same as the cell proliferation bag in Example 1, with the following differences:

[0052] The bottom surface of the conducting body is a rectangular parallelepiped with side lengths of 10×0.5×0.5 cm. There are two diverters, which are placed along the bottom third line of the bag.

[0053] like Figure 12 As shown, the tray 9 also includes filter support rods 17, the filter support rods 17 are two, spaced a certain distance in parallel and fixed on the tray 9, the gas filter 5 is placed on the filter support rods 17 , keep the hose from filter 5 to the first adapter tube upright.

[0054] like Figure 13 As shown in -14, the tray 9 also includes elastic bolts 18 (such as spring bolts, etc.), the elastic bolts 18 pass through the holes provided on the clamping plate 12, and abut on the fixing plate 19, so as to fix the tray 9 on the fixing plate 19. sports platform. When removing the tray, pull the elast...

Embodiment 3

[0056] The cell proliferation bag in this example is basically the same as the cell proliferation bag in Example 1, except that the bottom surface of the conducting fluid is a rectangle with a side length of 20×5cm, and its vertical section is a triangle with a height of 3cm. There are three guiding bodies, which are evenly distributed at the bottom of the bag body, and are placed in a staggered position at the three-point line of the bottom of the bag.

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Abstract

The invention discloses a cell proliferation bag and a cell proliferation device, and belongs to the technical field of cell proliferation. The proliferation bag comprises a bag body with the sealed edge. The bag body is provided with diversion bodies, the diversion bodies are fit with the bottom surface of the bag body, the side length or the diameter of the bottom surface of each diversion body is 0.5 to 20 cm, and the height of each diversion body is 0.5 to 10 cm. The cell proliferation device comprises a tray and the cell proliferation bag arranged on the tray, and each of two opposite side edges of the bag body of the cell proliferation bag is provided with a fixing rod arranged along the edge of the bag body; the tray is provided with clamping buckles clamped with the fixing rods; a tray load-bearing surface contacted with the cell proliferation bag is provided with projecting diversion bodies, the side length or the diameter of the bottom surface of each diversion bodies is 0.5 to 20 cm, and the height of each diversion body is 0.5 to 10 cm. With the arrangement of the diversion bodies, a culture solution in the cell proliferation bag is allowed to generate upward diversion when moving in a two-dimensional plane, so as to mix uniformly the culture solution under a small shear force.

Description

technical field [0001] The invention relates to the technical field of cell proliferation, in particular to a cell proliferation bag and a cell proliferation device. Background technique [0002] Cells are the most basic form of life, and no matter how deep the research on matter is, its impact on life is revealed at the cellular level. In order to study life topics and apply cell technology to benefit mankind, new cell technologies emerge one after another, reflecting the continuous development of modern biological technology. [0003] A complete cell process is a system that includes the selection of cell lines, the optimization of culture media, and the improvement of equipment. In recent years, due to the adoption of new development ideas, advanced cell proliferation processes have significantly improved work efficiency. With the development of modern material science, various equipments for disposable cell technology are constantly appearing. A modern cell technology ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/04
Inventor 吴琦
Owner GUANGZHOU ZHONGXI MEDICAL TECH
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