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Cell proliferation bag and cell proliferation device

A cell proliferation and bag body technology, applied in the field of cell proliferation, can solve the problems of inability to use active oxygen supply for cell proliferation, difficult to meet the needs of cell proliferation, and no air connection path, saving manpower, increasing pressure, and improving The effect of dissolved oxygen levels

Active Publication Date: 2015-03-11
GUANGZHOU ZHONGXI MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The passive method of natural infiltration is difficult to meet the needs of cell proliferation under high-density conditions
In the past, the cell proliferation bag did not have an air connection path, and could not use active oxygen supply to achieve cell proliferation
[0006] In the past, the cell proliferation bag used the liquid flow path on the side of the bag. In the actual culture state, these paths are directly in contact with the liquid below the liquid level, and the gas path cannot be connected.

Method used

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  • Cell proliferation bag and cell proliferation device
  • Cell proliferation bag and cell proliferation device
  • Cell proliferation bag and cell proliferation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A cell proliferation bag, such as figure 1 As shown in -3, it includes a bag body 1 with sealed edges; the bag body 1 is provided with a diversion body 2, and the diversion body is attached to the bottom surface of the bag body. The diameter of the bottom surface of the diversion body is 8 cm, and the height is 5 cm.

[0043] The bottom surface of the guide body is circular, and its vertical section is triangular, that is, the guide body is a cone.

[0044] There are three diversion bodies, which are evenly distributed on the bottom side of the bag body, and are misplaced at the third line of the bottom of the bag. The bag body is made of a soft film material with an ultra-high barrier to gas; Figure 4 As shown in -8, the top surface of the bag body is provided with a first transfer pipe 3 connecting the inside and outside of the bag body. There are two first transfer pipes 3, and the ends connected to the outside of the bag body are respectively connected to the gas ...

Embodiment 2

[0051] The cell proliferation bag of this embodiment is basically the same as the cell proliferation bag in Example 1, the difference is:

[0052] The bottom surface of the guide body is a cuboid with a side length of 10×0.5×0.5 cm. There are two deflectors, which are placed along the three-point line at the bottom of the bag.

[0053] like Figure 12 As shown, the tray 9 also includes filter support rods 17, the filter support rods 17 are two, fixedly placed on the tray 9 in parallel at a certain distance, and the gas filter 5 is placed on the filter support rods 17 , keep the rubber hose from the filter 5 to the first transfer pipe upright.

[0054] like Figure 13 As shown in -14, the tray 9 also includes an elastic bolt 18 (such as a spring bolt, etc.), the elastic bolt 18 passes through the hole provided on the clamping plate 12, and leans against the fixing plate 19, thereby fixing the tray 9 on the sports platform. When removing the tray, pull the elastic bolt outw...

Embodiment 3

[0056] The cell proliferation bag in this embodiment is basically the same as the cell proliferation bag in Example 1, except that the bottom surface of the guide body is a rectangle with a side length of 20×5 cm, and its vertical section is a triangle with a height of 3 cm. There are three diversion bodies, which are evenly distributed on the bottom of the bag body, and are misplaced at the three-point line at the bottom of the bag.

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Abstract

The invention discloses a cell proliferation bag and a cell proliferation device, and belongs to the technical field of cell proliferation. The proliferation bag comprises a bag body with the sealed edge. The bag body is provided with diversion bodies, the diversion bodies are fit with the bottom surface of the bag body, the side length or the diameter of the bottom surface of each diversion body is 0.5 to 20 cm, and the height of each diversion body is 0.5 to 10 cm. The cell proliferation device comprises a tray and the cell proliferation bag arranged on the tray, and each of two opposite side edges of the bag body of the cell proliferation bag is provided with a fixing rod arranged along the edge of the bag body; the tray is provided with clamping buckles clamped with the fixing rods; a tray load-bearing surface contacted with the cell proliferation bag is provided with projecting diversion bodies, the side length or the diameter of the bottom surface of each diversion bodies is 0.5 to 20 cm, and the height of each diversion body is 0.5 to 10 cm. With the arrangement of the diversion bodies, a culture solution in the cell proliferation bag is allowed to generate upward diversion when moving in a two-dimensional plane, so as to mix uniformly the culture solution under a small shear force.

Description

technical field [0001] The invention relates to the technical field of cell proliferation, in particular to a cell proliferation bag and a cell proliferation device. Background technique [0002] Cells are the most basic form of life. No matter how deep the study of matter is, its impact on life is revealed at the cellular level. In order to study life topics and apply cell technology to benefit mankind, new cell technologies emerge in an endless stream, reflecting the continuous development of modern biological technology. [0003] A perfect cell process is a system, which includes screening of cell lines, optimization of medium, and improvement of equipment. In recent years, due to the adoption of new development ideas, advanced cell proliferation technology has significantly improved work efficiency. With the development of modern material science, a variety of equipment for disposable cell technology has emerged. A modern cell technology represented by the bag system h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/04
Inventor 吴琦
Owner GUANGZHOU ZHONGXI MEDICAL TECH
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