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Preparation method for tissue engineering nerves of compound seed cells

A tissue engineering and nerve technology, applied in the field of tissue engineering of biomedical materials, can solve the problems of incomplete supply and limited source of allogeneic nerves, and achieve good nerve repair effect, obvious repair effect, and the effect of overcoming destructive effects.

Inactive Publication Date: 2015-07-01
居李生物科技(北京)有限公司
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Problems solved by technology

Although acellular allogeneic nerve transplantation has made some progress at home and abroad, the sources of allogeneic nerves are still limited, and the supply problem has not been completely resolved.

Method used

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  • Preparation method for tissue engineering nerves of compound seed cells
  • Preparation method for tissue engineering nerves of compound seed cells
  • Preparation method for tissue engineering nerves of compound seed cells

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preparation example Construction

[0055] The preparation method of tissue-engineered nerves of bone marrow mesenchymal stem cells induced by composite nerves provided by the present invention comprises the following operations:

[0056] 1) Take the peripheral nerve tissue of the isolated xenogeneic animal, remove the extraneural blood vessels, fat and connective tissue, and then treat it with trypsin; stop the digestion after the trypsin treatment is completed, and use the hypotonic treatment solution to perform hypotonic treatment on the nerve tissue. deal with;

[0057]2) Soak the hypotonic-treated nerve tissue in SB-10 solution for more than 12 hours, then transfer it to a mixed solution containing Triton X200 and SB-16 and soak for more than 12 hours, and obtain the decellularized nerve matrix after washing;

[0058] 3) Pre-induce the passaged bone marrow mesenchymal stem cells with DMEM-F12 medium containing 10-20% fetal bovine serum and 1-5 μmol / L β-mercaptoethanol for 12-24 hours, wash aseptically, and ...

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Abstract

The invention discloses a preparation method for tissue engineering nerves of mesenchymal stem cells induced by compound nerves. The preparation method for tissue engineering nerves of mesenchymal stem cells induced by compound nerves comprises the steps of extracting nerves with an improved enzyme-hypotension-chemical detergent in a trigeminy manner based on a Sondell preparation method, constructing tissue engineering peripheral nerves with biological activity in vitro, and then compounding mesenchymal stem cells which are subjected to in-vitro cultivation, amplification and neuronal differentiation induction. According to the preparation method provided by the invention, the destructive effect caused by an existing acceptable chemical preparation method, namely the Sondell method, on a nerve basal membrane tube and an extracellular matrix structure to a certain extent is overcome; the influence on axonal regeneration is alleviated. The tissue engineering nerves of the mesenchymal stem cells induced by the compound nerves, which are prepared by the method disclosed by the invention, can be applied to the preparation of nerve grafts and have good effects in restoring peripheral nerve defect.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and relates to a method for preparing tissue engineering nerves composed of bone marrow mesenchymal stem cells induced by nerves. Background technique [0002] Clinically, we often encounter peripheral nerve defects caused by tumor resection, trauma, etc., which cause muscle atrophy in patients' limbs and damage motor sensory functions. The treatment method is that if the distance of the defect is short, it can be dissected and sutured without tension. When the distance of the defect is too large, autologous nerve transplantation is usually required. Although autologous nerve transplantation has a certain curative effect and is the gold standard for comparison with other treatment methods, there are many shortcomings that are difficult to overcome. The most fatal shortcoming is that the choice of nerve donors is really limited. [1] . So far, there is no suitab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L27/38A61L27/36
Inventor 樊立宏王坤正党晓谦余泽锋
Owner 居李生物科技(北京)有限公司
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