A new HIV vaccine elicits a potent CD4+ T cell immune response

A technology for vaccines and purposes, applied in the field of vaccines

Inactive Publication Date: 2016-03-02
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A new HIV vaccine elicits a potent CD4+ T cell immune response
  • A new HIV vaccine elicits a potent CD4+ T cell immune response
  • A new HIV vaccine elicits a potent CD4+ T cell immune response

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Embodiment Construction

[0021] Below in conjunction with experiment, further illustrate the present invention.

[0022] The specific experimental methods not mentioned in the following experiments are usually carried out according to the existing conventional experimental methods.

[0023] In view of the lack of experimental models of HIV in the prior art, in the following experiments, an experimental model of SIV was used to evaluate the effect of the vaccine.

[0024] 1. Construction of the vaccine

[0025] Gene acquisition: The LC3b gene sequence was obtained from the NCBI database, and the LC3b gene was obtained through total gene synthesis. The gene sequence of LC3b is shown in SEQ ID NO: 1, and the gene sequence of SIVgag is shown in SEQ ID NO: 2; the pVAX vector carrying SIVgag and the Ad5-SIVgag recombinant adenovirus are preserved by our laboratory.

[0026] vaccine construction

[0027] The insertion sequence of the foreign element of the plasmid vector is as follows: figure 1 As shown ...

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Abstract

The invention discloses a potent CD4+? A new type of HIV vaccine for T cell immune response, the LC3b gene is fused with the HIV antigen gene, and then the fusion gene is cloned into a vaccine vector to construct a new vaccine. According to the animal experiment data, it can be predicted that compared with HIV vaccines of other technologies, the HIV vaccine designed using the method of the present invention can induce more intense, more broad-spectrum and more multifunctional T lymphocytes, especially multifunctional T lymphocytes. Functional CD4+? T cell response, this approach can also induce a strong B cell response. The technology of the invention can be generally applied to the prevention and treatment of AIDS.

Description

technical field [0001] The present invention relates to a vaccine, in particular to a novel HIV vaccine capable of inducing strong CD4+ T cell immune response. Background technique [0002] Since the discovery of AIDS (Acquired Immune Deficiency Syndrome, AIDS) in 1984, great progress has been made in the treatment and prevention of AIDS in the past 30 years. However, AIDS remains one of the most serious diseases threatening people's health globally. By the end of 2012, about 34 million people worldwide were infected with AIDS, and the cumulative death toll exceeded 30 million. [0003] So far, no effective HIV vaccine has been used clinically. For a long time, the specific cellular immune response induced by HIV, especially the CD8+ T cell immune response, was considered to be the main control of HIV virus replication. Therefore, many HIV vaccine designs are based on this point, inducing a stronger CD8+ T cell response to reduce the viral load. However, the failure of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/21A61P31/18
Inventor 孙彩军陈凌靳怡
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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