Method for breeding fast growing type black-skin chicken strain

A breeding method and technology for black-skinned chickens, applied in the direction of animal husbandry, etc., can solve the problems of the size of the expansion group, the long period, the cumbersome test-crossing process, etc., to speed up the breeding process, the carcass looks beautiful, and overcome the cumbersome test-crossing process. Effect

Active Publication Date: 2015-04-22
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the test cross process is cumbersome, the cycle is long, and it will be affected by the size of the expanded group

Method used

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  • Method for breeding fast growing type black-skin chicken strain

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Embodiment 1

[0030] The breeding method of fast large-scale black-skinned chicken strain in the present embodiment, comprises the following steps:

[0031] (1) Use silky silkie roosters as the male parent and Ross 308 hen as the female parent to perform crossbreeding to produce F1 generation chickens, and select F1 generation black-skinned chickens;

[0032] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation black-skinned chicken is selected;

[0033] (3) Determine the relative copy number of the EDN3 gene of the F2 generation of black-skinned chickens by fluorescent quantitative PCR, and determine the genotype of the F2 generation of black-skinned chickens, specifically including the following steps:

[0034] ① Collection of samples

[0035] Take blood from the black-skinned chicken wings selected from the F2 generation, add 1 / 3 anticoagulant, and extract blood DNA by proteinase K method;

[0036] ② DNA sample concentration adjustment

[0037] Use ...

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Abstract

The invention discloses a method for breeding fast growing type a black-skin chicken strain, and belongs to the technical field of molecular breeding. According to the method disclosed by the invention, a molecule-assisted selection method is adopted to identify the genotype of the black-skin locus of the chicken strain, the genotype of the locus can be rapidly determined, and the defects of tedious test cross, long generation interval and the like in the traditional conventional breeding are overcome. The black-skin chicken strain of which the genotype is FmFm is produced by using the method disclosed by the invention, the tedious test cross breeding is avoided, the generation interval is shortened, the breeding process is quickened, and the breeding cost is reduced; furthermore, the black skin character of the strain is uniform, the carcass is more attractive in appearance the meat is tender, and the nutritive value is high; in addition, when the strain is used as the male parent to hybrid-mate a fast growing type white feather broiler, the black-skin chicken high in growing performance can be produced.

Description

technical field [0001] The invention specifically relates to a method for cultivating a fast large black-skinned chicken strain, and belongs to the technical field of molecular breeding. Background technique [0002] Silkie is a well-known medicinal poultry at home and abroad. It was recognized as a standard breed internationally in 1874. It has special medicinal, nutritional and ornamental values. And foreign fast large white feather broiler grows fast. In order to improve its growth performance, breeders often need to introduce bloodlines of fast and large broilers in breeding practice, which will cause the segregation of the black skin trait. The medicinal value of black-bone chicken mainly lies in the effect of its melanin, and the trait of black-bone skin is the most direct judgment of consumers on the expression of melanin. In order to maintain the traits of black skin while providing growth performance, it is very important to determine the genotype of the black ski...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
Inventor 康相涛杨朋坤韩瑞丽田亚东李转见王丹丹孙桂荣蒋瑞瑞吕世杰王彦彬刘小军李国喜闫峰宾黄艳群许殿明李孝法索智勇张瑞勤
Owner HENAN AGRICULTURAL UNIVERSITY
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