A Breeding Method of Blue-shank Recessive White Feather Chicken Strain Based on Molecular Assisted Selection
A recessive white-feathered chicken, molecular-assisted technology, applied in animal husbandry, etc., can solve the problems of slow growth, poor meat quality, low feed conversion rate, etc., and achieve the effect of tender meat, avoiding tediousness, and delicious meat
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[0034] The breeding method of the blue-shin recessive white-feathered chicken strain based on molecular assisted selection in the present embodiment comprises the following steps:
[0035] (1) Using the Qingshan recessive white feather chicken as the male parent and the white Laihang chicken as the female parent in the Gushi chicken population to perform crossbreeding to produce the F1 generation;
[0036] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation of green shin and white feather male and female chickens are selected;
[0037] (3) Using the RFLP-PCR method of artificially introducing PvuII to determine the genotype of the F2 generation of blue-shined white-feathered male and female hens, specifically including the following steps:
[0038] ① Collection of samples
[0039] Take blood from the veins of the chicken wings with green shanks and white feathers selected from the F2 generation, add 1 / 3 anticoagulant, extract blood DNA wit...
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