Method for cultivating dominant-green-shin white feather broiler strain based on molecular assistant selection

Abstract

The invention discloses a method for cultivating a dominant-green-shin white feather broiler strain based on molecular assistant selection and belongs to the technical field of molecular breeding. According to the method for cultivating the dominant-green-shin white feather broiler strain based on molecular assistant selection, the method of molecular assistant selection is adopted for appraisal of a genotype of a broiler dominant white feather locus, so that the genotype of the white feather locus is rapidly judged, the dominant-green-shin white feather broiler strain with the II genotype is produced, complexity of test cross selective breeding is avoided, a generation interval is shortened, the breeding progress is accelerated, and therefore the cultivation cost is reduced. Meat of the dominant-green-shin white feather broiler strain cultivated through the method is fine and tender, taste of the meat of the broiler strain is delicious, the production performance of the broiler strain is high, color feather residues do not exist on a carcass, and therefore the carcass is beautiful. In addition, the strain can serve as a male parent and be hybridized with a high-yield laying hen strain or a fast-growing large broiler strain in a matched mode so that high-yield or fast-growing large dominant-green-shin white feather broilers can be produced respectively.

Description

technical field

[0001] The invention specifically relates to a method for cultivating a blue-shin dominant white-feathered broiler strain based on molecular assisted selection, and belongs to the technical field of molecular breeding. Background technique

[0002] my country's local chicken breeds are rich in resources, and its meat is tender and delicious, but it is mostly colored feathers such as yellow feathers and hemp feathers in appearance. For the chilled fresh chicken market, colored feather chickens often affect the appearance of the carcass because the feathers are not easy to remove after slaughter. For foreign fast and large white-feathered broiler chickens, the growth rate is fast and the carcass properties are good, but the meat quality is far inferior to that of local chickens in our country. Therefore, it is of great significance in the cultivation of dominant white feather high-quality broilers.

[0003] In traditional breeding, the method of test crossing...

Examples

Embodiment 1

[0035] The breeding method of the blue-shin dominant white-feathered broiler strain based on molecular assisted selection in the present embodiment comprises the following steps:

[0036] (1) Use Gushi pure line roosters as the male parent and Avian hen as the female parent to carry out crossbreeding to produce F1 generation chickens, eliminate a small number of individuals with colored feathers that appear in the hybridization, and select the F1 generation of blue feathers from the F1 white feather population. White-shined male and female hens;

[0037] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation of green shin and white feather male and female chickens are selected;

[0038] (3) Using the RFLP-PCR method of artificially introducing PvuII to determine the genotype of the F2 generation of blue-shined white-feathered male and female hens, specifically including the following steps:

[0039] ① Collection of samples

[0040] Take bloo...

technical field

[0001] The invention specifically relates to a method for cultivating a blue-shin dominant white-feathered broiler strain based on molecular assisted selection, and belongs to the technical field of molecular breeding. Background technique

[0002] my country's local chicken breeds are rich in resources, and its meat is tender and delicious, but it is mostly colored feathers such as yellow feathers and hemp feathers in appearance. For the chilled fresh chicken market, colored feather chickens often affect the appearance of the carcass because the feathers are not easy to remove after slaughter. For foreign fast and large white-feathered broiler chickens, the growth rate is fast and the carcass properties are good, but the meat quality is far inferior to that of local chickens in our country. Therefore, it is of great significance in the cultivation of dominant white feather high-quality broilers.

[0003] In traditional breeding, the method of test crossing...

Examples

Embodiment 1

[0035] The breeding method of the blue-shin dominant white-feathered broiler strain based on molecular assisted selection in the present embodiment comprises the following steps:

[0036] (1) Use Gushi pure line roosters as the male parent and Avian hen as the female parent to carry out crossbreeding to produce F1 generation chickens, eliminate a small number of individuals with colored feathers that appear in the hybridization, and select the F1 generation of blue feathers from the F1 white feather population. White-shined male and female hens;

[0037] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation of green shin and white feather male and female chickens are selected;

[0038] (3) Using the RFLP-PCR method of artificially introducing PvuII to determine the genotype of the F2 generation of blue-shined white-feathered male and female hens, specifically including the following steps:

[0039] ① Collection of samples

[0040] Take bloo...

Images