A kind of breeding method of high-yielding black-skinned chicken strain

A breeding method and technology of black-skinned chickens, applied in animal husbandry and other fields, can solve the problems of size influence of colony expansion, long cycle, cumbersome test cross process, etc., to achieve accelerated breeding process, high production performance, and overcome cumbersome test cross problems Effect

Active Publication Date: 2016-01-20
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the test cross process is cumbersome, the cycle is long, and it will be affected by the size of the expanded group

Method used

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  • A kind of breeding method of high-yielding black-skinned chicken strain

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Embodiment 1

[0030] The breeding method of high-yield type black-skinned chicken strain in the present embodiment, comprises the following steps:

[0031] (1) Using silky silkie pure line cock as the male parent and Bailai aircraft carrier chicken as the female parent to carry out crossbreeding to produce F1 generation chickens;

[0032] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation black-skinned chicken is selected;

[0033] (3) Determine the relative copy number of the EDN3 gene of the F2 generation of black-skinned chickens by fluorescent quantitative PCR, and determine the genotype of the F2 generation of black-skinned chickens, specifically including the following steps:

[0034] ① Collection of samples

[0035] Take blood from the black-skinned chicken wings selected from the F2 generation, add 1 / 3 anticoagulant, and extract blood DNA by proteinase K method;

[0036] ② DNA sample concentration adjustment

[0037] Use a micro-spectrophotom...

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Abstract

The invention discloses a method for cultivating a high-yield black-skinned chicken strain, and belongs to the technical field of molecular breeding. The invention adopts the molecular-assisted selection method to identify the genotype of the black skin locus, can quickly determine the genotype of the locus, and overcomes the shortcomings of cumbersome cross-testing and long generation intervals in traditional conventional breeding. Adopting the method of the present invention to produce the high-yield black-skinned chicken strain whose genotype is FmFm shortens the generation interval, accelerates the breeding process, and reduces the cultivation cost; moreover, the egg-producing performance of the strain is good, and the nutritional value of eggs is high; The skin properties are uniform, and the carcass is more beautiful. In addition, using this line as a male parent to crossbreed with high-yielding laying hens can produce black-skinned chickens with higher production performance.

Description

technical field [0001] The invention specifically relates to a method for cultivating a high-yield black-skinned chicken strain, and belongs to the technical field of molecular breeding. Background technique [0002] Black-bone chicken is a well-known medicinal poultry at home and abroad. It was recognized as a standard breed internationally in 1874. Black-bone chicken and black-bone eggs have special medicinal, nutritional and ornamental values. But the egg production performance of black-bone chicken is low. The laying performance of foreign high-yield laying hens is very good. In order to improve its production performance, breeders often need to introduce bloodlines of high-yield laying hens in breeding practice, which will cause the separation of black skin traits, and then affect the price of culled hens. In order to maintain the black skin trait of culled hens while improving production performance, it is very important to determine the genotype of the black skin lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
Inventor 康相涛田亚东杨朋坤韩瑞丽王丹丹李转见孙桂荣蒋瑞瑞吕世杰闫峰宾王彦彬刘小军李国喜黄艳群郭晗许殿明李孝法索智勇
Owner HENAN AGRICULTURAL UNIVERSITY
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