Method for induction and diploidization of different-germplasm haploid of corn in northwest Guizhou

A haploid induction, haploid technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., to achieve the effect of simple haploid

Inactive Publication Date: 2014-06-04
毕节市农业科学研究所
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Problems solved by technology

[0004] At present, the method of induction rate of maize haploid in northwestern Guizhou and the use of different concentrations of colchicine to obtain the best combination of methods suitable for haploid diploidization of maize in northwestern Guizhou are not yet mature.

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Embodiment Construction

[0022] Hereinafter, the above-mentioned and other technical features and advantages of the present invention will be described in more detail.

[0023] The inducible line of the present invention Nongda No. 1 and B73 and Northwest Guizhou mainly use excellent inbred lines of corn and the F2 generation hybridization of Bidan 17 and Bidan 18 to count the induction rate of different corn haploids, and to explore the improvement of corn haploids in Northwest Guizhou The method of induction rate; using different concentrations of colchicine, at different temperatures and processing times, the best combination of methods suitable for the diploidization of corn haploids in Northwest Guizhou was obtained, and the percentage of successful doubling was calculated to construct a BiDan 17. The F2 generation of Bi Dan 18 corresponds to the DH line, preparing for the follow-up basic research.

[0024] Step a, the acquisition of haploid seeds: use Nongda Gaoyu No. 1 as the male parent, and h...

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Abstract

The invention relates to a method for induction and diploidization of different-germplasm haploids of corn in northwest Guizhou. The method comprises the following steps: respectively hybridizing an Agriculture university high-induction No.1 as a male parent with different systems so as to obtain haploid seeds of different systems, respectively multiplying by using a seed seeking method, a root seeking method and a bud seeking method, treating for different times at different temperatures, counting the diploidization success rates of different systems in different treatments so as to obtain an optimal multiplying method appropriate to main excellent corn systems in northwest Guizhou, performing seeding experiment, recording successful diploidization planting in field, analyzing different-germplasm inductivity and diploidization, wherein the percentage of the number of haploid seeds to the total number of the seeds is just the different-germplasm inductivity, performing field investigation to obtain the percentage of the number of diploid plants to the number of the haploid seeds, that is, the diploidization success rate, and establishing a corresponding DH system. By adopting the method, a haploid breeding technique system for the characteristic of the corn in northwest Guizhou is established, fine genes are combined together, and theoretic reference is provided for breeding of novel high-production and high-resistance varieties.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for inducing haploids and diploidization of different germplasms of maize in northwest Guizhou. Background technique [0002] The corn growing season in Northwest Guizhou is high temperature, high humidity, serious pests and diseases, climate changeable, and poor soil. Therefore, varieties that are resistant to early drought, resistance to diseases and insect pests are welcomed by customers. Through the efforts of generations of breeders, many inbred lines and varieties with excellent resistance have been bred. For example, the inbred line y054 bred by Bijie Agricultural Science Institute not only has excellent phenotype, but also has excellent resistance. [0003] At present, in maize haploid breeding, the doubling rate is still low due to natural and other methods, and chemical doubling is mostly used for doubling. During the growth and development of haploid plants, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 李清超张时龙文琼梁黔云梅艳王嵩刘建新马俊先新李雪松孙开利
Owner 毕节市农业科学研究所
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