Construction method of Adriamycin nephropathy animal model
An animal model and construction method technology, which can be used in pharmaceutical formulations, drug combinations, urinary system diseases, etc., can solve the problems of increasing rat mortality, affecting modeling effects, and high model mortality, achieving short and consistent modeling time. Good performance and stability, easy to operate
Inactive Publication Date: 2014-06-18
河南省医药科学研究院
View PDF0 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Because doxorubicin has strong toxic and side effects on the heart, liver, bone marrow, gastrointestinal system and other systems, a single high-dose injection makes the mortality rate of this model high, although the measures of shortening the modeling time and reducing the dosage of doxorubicin can Improve the survival rate of the model, but it will affect the modeling effect
Some people also use the method of right nephrectomy and repeated injection of ADR to make models, but in the experiment, rats will die due to surgical accidents or infection, and the death rate of rats will increase after injection of ADR
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0014] Example 1: Construct an animal model of adriamycin nephropathy according to the following steps:
[0015] (1) Six-week-old SPF male SD rats weighing 130~150g were selected, and after one week of adaptive feeding, routine urine test was performed, and the test results were normal (urine protein was negative) and 70 rats weighing 180~200g The rats were randomly divided into the control group A and the model group; among them, the control group A was 7 rats, and the remaining 63 were model groups; the rats in the model group were randomly divided into the normal feed group B group And the high protein feed group C group; among them, there were 31 rats in the B group and 32 rats in the C group.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a construction method of an Adriamycin nephropathy animal model, for solving the technical problem that an existing Adriamycin nephropathy animal model is complex in operation and low in success rate. The construction comprises the following steps: selecting rats according with experimental standards as a molding group; injecting Adriamycin to the rats of the molding group by caudal vein for the first time according to a using amount of 4mg / kg; one week later, injecting Adriamycin for the second time according to the using amount of 3.5mg / kg; from the first injection of Adriamycin to the rats in the molding group, feeding the rats with high protein feed for two weeks, and then feeding with a normal feed for 6 days; and from the first injection of the Adriamycin to the 20th day, reckoning the rats with the total amount of 24-hour urine protein being larger than 30mg into the Adriamycin nephropathy animal model. The construction method disclosed by the invention is simple, feasible, high in success rate, good in consistency and stability and capable of effectively reducing the animal death rate and is a relatively ideal animal model for further researching sertoli cytopathy.
Description
Technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of adriamycin nephropathy. Background technique [0002] Adriamycin (ADR) is a quinone-containing anthracycline antibiotic, which can be metabolized and reduced in the kidney to semiquinone free radicals. The latter reacts with oxygen to generate reactive oxygen species, induce lipid peroxidation in glomerular epithelial cells, and affect sugar Protein metabolism destroys the structure and function of the filtration membrane, and ultimately leads to the selective change of the membrane filtration barrier, causing proteinuria. The nephrotoxic effect of adriamycin and the stimulation of a large amount of proteinuria further induce the production and release of various cytokines and inflammatory mediators in glomerular cells and other inflammatory cells, and stimulate the proliferation and mesangium of glomerular mesangial cells...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P13/12A01K67/027
Inventor 何美霞贺石林赵瑛瑛王巨才张勇朱建立李杨李志刚何叶张翰明杜斌华海婴张卫茹张莉蓉
Owner 河南省医药科学研究院