Antigen-specific immature DC (dendritic cell) source exosome as well as preparation and application methods thereof
An application method and specific technology, applied in the direction of medical materials derived from mammals, animal cells, neuromuscular system diseases, etc., can solve the problem of non-antigen-specific, antigen-specific treatment of autoimmune diseases, and inability to achieve treatment Effect and other issues
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[0037] The following in conjunction with specific embodiments is intended to further illustrate the present invention, rather than limit the present invention.
[0038] 1. Preparation of antigen-specific immature DC-derived exosomes
[0039] 1.1 Isolation and purification of myeloid-derived immature DC and phenotypic detection
[0040] Select 6-8 week old B6 mice, take the femur and tibia under sterile conditions, lyse the bone marrow fluid of the tibia and fibula with red blood cells to remove red blood cells, then add RPMI1640 complete medium containing 10ng / ml rmGM-CSF to culture for 72 hours and then the full amount The culture was continued until the fifth day after changing the medium, and then cultured until the seventh day after changing the medium in half. According to the instructions of magnetic bead sorting, select anti-CD11c magnetic beads to sort and purify DC, so that the purity can reach more than 95% (attached figure 1 ).
[0041] 1.2 Flow cytometry detecti...
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