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Immunoassay method and immunoassay apparatus

A technology for immunoassay and object determination, which is applied in the direction of measuring devices, scattering characteristic measurement, instruments, etc., and can solve problems such as measurement result errors

Inactive Publication Date: 2014-08-13
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this measurement method, if the sample contains substances other than the measurement target such as chylomicrons, the measurement result will be inaccurate

Method used

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  • Immunoassay method and immunoassay apparatus
  • Immunoassay method and immunoassay apparatus
  • Immunoassay method and immunoassay apparatus

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Embodiment Construction

[0126] Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0127] In this embodiment, the present invention is applied to an immunoassay method and an immunoassay device for detecting aggregation of a substance to be measured and carrier particles mixed by a particle agglutination method, and quantifying the substance to be measured based on the detection result.

[0128] In the particle agglutination method, a sample containing a substance to be measured and carrier particles sensitized with an antibody or antigen corresponding to the substance to be measured are first mixed. If the substance to be measured exists in the sample, the carrier particles will aggregate due to the antigen-antibody reaction. As for the antibody or antigen sensitized by the carrier particles, if the substance to be measured is an antibody, an antigen that undergoes a specific antigen-antibody reaction with the antibody is us...

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Abstract

The invention provides an immunoassay method and an immunoassay apparatus capable of further reducing errors due to non-target substances. A detector measures a measurement specimen and detects information regarding the binding number of carrier particles included in the measurement specimen. A controller classifies, based on the information regarding the binding number, particles included in the measurement specimen into groups, the groups being classified in accordance with the binding numbers. Further, for each classified group, the controller performs either one of a first removing process for removing, from a processing target, data of non-target substances different from the carrier particles, and a second removing process for removing, from the processing target, data of the non-target substances through a process different from the first removing process, and obtains information regarding the agglutination degree of the carrier particles, based on data of the carrier particles obtained by performing the removing process.

Description

technical field [0001] The present invention relates to an immunoassay method and an immunoassay device using particle agglutination. Background technique [0002] An immunoassay method using Counting Immunoassay (CIA) is known. In this immunoassay method, carrier particles sensitized to an antigen or antibody corresponding to a substance to be measured are mixed with a sample to prepare a measurement sample, and the prepared measurement sample is measured to obtain the degree of aggregation of the carrier particles. In this measurement method, if substances other than the measurement target such as chylomicrons are contained in the sample, errors will occur in the measurement results. Known techniques for reducing such errors include, for example, those described in U.S. Patent No. 5,527,714 and U.S. Patent Application Publication (U.S. Patent Application Publication) No. 2005 / 148099. [0003] According to the technology described in U.S. Patent (U.S. Patent) No. 5,527,71...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/47
CPCG01N33/5302G01N21/4788G01N33/54313G01N21/49
Inventor 越村直人
Owner SYSMEX CORP
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