Moschus anhuiensis microsatellite loci, primers and application of primers
A microsatellite locus and satellite technology, applied in Anhui musk deer microsatellite loci, primers and their application fields, can solve the problems of difficulty in sample collection, difficult to find, and the scarcity of Anhui musk deer populations.
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[0012] The present invention will be described in detail below in conjunction with specific embodiments.
[0013] 1. DNA extraction for library construction
[0014] Since high-quality templates are required for library construction and screening of microsatellite sites, we clipped Anhui musk deer muscle tissue (from poached samples collected by the Forestry Bureau) and extracted DNA as templates for the microsatellite enrichment library. Genomic DNA was extracted using SDS / proteinase K cleavage and phenol-chloroform extraction (Sambrook et al., 1999).
[0015] 2. Construction of Anhui Musk Deer Microsatellite Enrichment Library
[0016] Genomic DNA was extracted, and AFLP rapid isolation method (Fast Isolation by AFLP of Sequences Containing repeats, FIASCO) was used to construct a microsatellite enrichment library. The specific process was as follows: about 250 ng of genomic DNA was taken, digested with MseI, and simultaneously treated with AFLP of MseI. Body linker (5'-TA...
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