Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Hyla tsinlingensis microsatellite sites, primers for microsatellite sites and application of microsatellite sites and primers

A technology of microsatellite sites and hyla, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve problems such as blanks

Inactive Publication Date: 2015-02-04
ANHUI UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, research in this area is still blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hyla tsinlingensis microsatellite sites, primers for microsatellite sites and application of microsatellite sites and primers
  • Hyla tsinlingensis microsatellite sites, primers for microsatellite sites and application of microsatellite sites and primers
  • Hyla tsinlingensis microsatellite sites, primers for microsatellite sites and application of microsatellite sites and primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] The present invention will be described in detail below in conjunction with specific embodiments.

[0009] 1. DNA extraction

[0010] Genomic DNA of Hyla Qinling was extracted using muscle or blood tissue. Genomic DNA was extracted using SDS / proteinase K cleavage and phenol-chloroform extraction (Sambrook et al., 1999).

[0011] 2. Construction of microsatellite enrichment library of Hyla Qinling

[0012] Genomic DNA was extracted, and AFLP rapid isolation method (Fast Isolation by AFLP of Sequences Containing repeats, FIASCO) was used to construct a microsatellite enrichment library. The specific process was as follows: Take about 250ng of genomic DNA, digest it with MseI, and at the same time combine it with the AFLP receptor of MseI Connector (5'-TAC TCA GGA CTC AT-3' / 5'-GAC GAT GAG TCC TGA G-3') for connection. After diluting the digested product tenfold, use AFLP receptor-specific primers (5'-GAT GAG TCC TGA GTA AN-3', referred to as MseI-N) for PCR amplificatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Hyla tsinlingensis microsatellite sites, primers for the microsatellite sites and application of the microsatellite sites and the primers. The Hyla tsinlingensis microsatellite sites include 12 stable polymorphic microsatellite sites of which the sequences are respectively shown in SEQ ID NO:1-SEQ ID NO:12. By using the Hyla tsinlingensis microsatellite sites and through analyzing genetic typing results of 18 Hyla tsinlingensis individuals, the number of alleles is 3-12, expected heterozygosity He is 0.145-0.903, observed heterozygosity Ho is 0-0.579. All the sites show relatively high polymorphism and the requirement for individual identification is reached.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to microsatellite sites, primers and applications of Hyla qinlingensis. Background technique [0002] Qinling hyla (Hyla tsinlingensis) belongs to the genus Hyla genus Hyla. It is an endemic species in China and has a wide distribution range. It is distributed in Yuexi County and Huoshan County of Anhui Province, southern Shaanxi, southern Gansu and Chongqing. In the context of global amphibians being threatened, it is imperative to strengthen the protection of amphibians. Molecular biological information provides a certain theoretical basis for the establishment of conservation units for modified species. However, the research in this area is still blank. Contents of the invention [0003] The invention discloses 12 microsatellite sequences and 12 pairs of microsatellite primers of Hyla Qinling. These loci play an important role in the conservation genetics research, germplasm res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 张保卫康兴王慧
Owner ANHUI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products