Novel body cell reprogramming inducing method, kit and application

A kit and reprogramming technology, applied in the field of inducing somatic cell reprogramming, can solve problems such as no reports of effects

Active Publication Date: 2014-10-29
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GMNN has been widely reported as an ectodermal differentiation gene, but its role in reprogramming has not been reported so far

Method used

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  • Novel body cell reprogramming inducing method, kit and application
  • Novel body cell reprogramming inducing method, kit and application
  • Novel body cell reprogramming inducing method, kit and application

Examples

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Embodiment Construction

[0041] The invention is further illustrated by the examples. Those of ordinary skill in the art can understand that the present invention is not limited to the embodiments, and those of ordinary skill in the art can modify the embodiments based on the teaching of the specification. These modifications are also included within the scope of the present invention as defined by the appended claims.

[0042] The experimental methods in the following examples are conventional methods unless otherwise specified.

[0043] 1. Mouse Strains

[0044] Transgenic mouse strain: TgN(GOFGFP)11Imeg(OG) was purchased from RIKEN BioResource Center. The mouse strain ICR was purchased from Victoria River.

[0045] 2. Cell Culture

[0046] Primary mouse embryonic fibroblasts (MEFs) and adult mouse skin cells (MDFs) were derived from ICR×OG transgenic mice. The culture medium for MEF and 293T cells was DMEM (Hyclone) medium containing 10% fetal bovine serum (FBS, Invitrogen). Mouse embryonic s...

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Abstract

The invention provides a novel body cell reprogramming inducing method, a kit and an application and relates to an application of an ectodermal differentiation and development correlation factor and/or mesendodermal differentiation and development correlation factor as an inducing factor to generation of an induced pluripotent stem cell. According to the invention, the inducing factor is provided for a differentiated stem cell to induce to generate the induced pluripotent stem cell (iPS cell), and the inducing factor comprises the mesendodermal differentiation and development correlation factor, Sox2, Klf4 and any one c-Myc or the ectodermal differentiation and development correlation factor, Oct4, Klf4 and any one c-Myc or the mesendodermal differentiation and development correlation factor, the ectodermal differentiation and development correlation factor, Klf4 and any one c-Myc. The iPS cell obtained by using the novel body cell reprogramming inducing method has characteristics similar to those of a rat embryonic stem cell, namely has self-renewal maintaining capacity and differentiation omnipotency.

Description

technical field [0001] The invention relates to a method for inducing somatic cell reprogramming, a kit and application. Background technique [0002] (1) Introduction to embryonic stem cells [0003] Embryonic stem cells (ESCs, referred to as ES cells.) Embryonic stem cells are a type of cells isolated from early embryos (before the gastrula stage), which have the characteristics of unlimited proliferation, self-renewal and multi-directional differentiation in vitro. [0004] (2) Characteristics of stem cell pluripotency: [0005] Embryonic stem cells are called "pluripotent" stem cells because they have the ability to differentiate into three germ layer cells. In addition, embryonoma (EC) cells and embryonic germ (EG) cells have similar pluripotency to embryonic stem cells. The common characteristics of pluripotent stem cells are: ES cells have a morphological structure similar to that of early embryonic cells, with large nuclei, one or several nucleoli, mostly euchromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10
Inventor 邓宏魁赵扬舒健吴晨吴业涛
Owner PEKING UNIV
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