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Marker for carrying out early-stage molecule screening of breast cancer by utilizing two methylation sites in control region of gene DFNA 5

A technology of gene regulation and methylation, applied in recombinant DNA technology, DNA/RNA fragment, determination/inspection of microorganisms, etc., can solve the problems of chromosomal instability and increase the chance of mutation

Inactive Publication Date: 2014-11-19
NANJING MAIDA PHARMA TECH CO LTD
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Problems solved by technology

Evidence shows that hypomethylation of the genome can lead to chromosomal instability and increased mutation opportunities, and the overall methylation status in the cell may be a contributing factor to the initiation of cancer

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  • Marker for carrying out early-stage molecule screening of breast cancer by utilizing two methylation sites in control region of gene DFNA 5

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Embodiment Construction

[0021] DNA methylation: It is an epigenetic modification that converts cytosine into 5-methylcytosine by DNA methyltransferase catalyzing S-adenosylmethionine as a methyl donor, commonly found at the 5′ of genes - CG-3' sequence, which plays an important role in normal human development, X chromosome inactivation, aging, tumors and many disease processes.

[0022] Infinium Methylation Assay: Use bisulfite to convert cytosine → uracil to detect whether gDNA is methylated. After bisulfite acts on gDNA, the unmethylated cytosine in gDNA will be converted into uracil, while the methylated cytosine will remain the same. Two site-specific probes designed by Infinium Methylation Assay, one paired with methylated sites and one paired with unmethylated sites, the paired probes were extended by single base, and then stained with fluorescent reagents, The degree of gDNA methylation can be judged by the captured fluorescent signal.

[0023] Day1:

[0024] Start Bisulfite Conversion

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Abstract

The invention provides a method for carrying out early-stage molecule screening of breast cancer by utilizing two methylation sites in a control region of a gene DFNA 5. In a methylation microarray data analytical process on two breast cancer cell lines, results show that methylation of two CpGs in the control region of the gene DFNA 5 is obviously lower than that of other genes, the demethylation of the gene DFNA 5 is supposed to be one important characteristic of pathogenesis of the breast cancer, and the two methylation sites can be used as one marker used for early-stage screening of the breast cancer. The marker provided by the invention can be possibly a cheap marker used for carrying out early-stage molecular screening of the breast cancer.

Description

Technical field [0001] The invention relates to a marker for early molecular screening of breast cancer. Two methylation sites in the regulatory region of DFNA5 gene can be used as an early warning marker for breast cancer early screening. Background technique [0002] Breast cancer is the most common malignant tumor in women all over the world. Regular mammography examination can reduce the mortality rate of breast cancer in women over 40 years old, so it is the only effective breast screening measure so far. Although mammography has high application value in the early detection of breast cancer, it may have missed diagnosis, and its application value in judging benign and malignant abnormal lesions is also very limited. There is no effective way to distinguish whether the occurrence of breast cancer is a missed diagnosis or a new occurrence. Therefore, a simple, rapid and cheap method for early screening of breast cancer will benefit hundreds of millions of women in Chin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q1/6883C12Q2600/154
Inventor 李艾斯
Owner NANJING MAIDA PHARMA TECH CO LTD
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