Multichannel fluorescent PCR detection kit for congenital deafness gene
A technology of congenital deafness and detection kits, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems that are not suitable for clinical application, prevent false negatives, avoid mutual interference, and reduce hydrolysis pressure Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0057] Example 1: Composition and detection method of a congenital deafness gene multi-channel fluorescent PCR detection kit
[0058] 1. The composition of the kit:
[0059] 1 tube of DNA extraction solution (5.0ml), 1 tube of PCR reaction solution (1.1ml), 1 tube of negative quality control (50μl), 1 tube of positive quality control (50μl);
[0060] The composition of each 50 μl PCR reaction solution is: 5.0 μl of 10×PCR buffer, MgCl 2 3.0mM, dNTPs 1.0mM, primer 1.0μM, probe 0.5μM, Taq enzyme 3.0U, UNG enzyme 1.0U, add water to 50μl.
[0061] Wherein, the primers are primer 1, primer 2, primer 3 and internal reference primers, and their sequences are respectively:
[0062] Primer 1F: 5'-ATGGATTGGGGCACGCTG-3' (SEQ ID NO: 1),
[0063] Primer 1R: 5'-GACCTTCTGGGTTTTGAT-3' (SEQ ID NO: 2)
[0064] Primer 2F: 5'-GTACGACGGCTTCTCCAT-3' (SEQ ID NO: 3)
[0065] Primer 2R: 5'-GACAGTCTTTCTCCGTGGG-3' (SEQ ID NO: 4)
[0066] Primer 3F: 5'-CTCTTCCTCTACCTGCTGC-3' (SEQ ID NO: 5)
[0067...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com