Microbiological detection method
A microbial detection and microbial technology, applied in the field of microbial detection, can solve the problems that the results cannot be obtained quickly, and the production of food industry cannot be satisfied.
Inactive Publication Date: 2015-07-22
安徽信灵检验医学科技股份有限公司
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Problems solved by technology
However, the cultivation method still needs to be at the cost of time, and the results cannot be obtained quickly, which is far from meeting the needs of food industry production and environmental health supervision on-site testing
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 2
[0019] The rest are the same as in Example 1, except that the pH of the culture solution in the step (1) is 7.5; the temperature of the microorganism culture in the step (2) is 36 degrees, and the culture time is 3 hours.
Embodiment 3
[0021] The rest is the same as that of Example 1, except that the pH of the culture solution in the step (1) is 7; the microorganism culture temperature in the step (2) is 37 degrees, and the culture time is 6 hours.
[0022] After the above-mentioned process steps, the obtained microbial detection results are that both Examples 1 and 3 emit light, and microorganisms can be detected, and there is no microorganism in Example 2.
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The invention discloses a microbiological detection method. The microbiological detection method comprises the following steps: (1) preparing a culture medium; (2) culturing microorganism; (3) extracting ATP (Adenosine Triphosphate) from microbial cells; (4) performing luminescence detection; (5) identifying. The microbiological detection method disclosed by the invention is used for detecting whether a sample contains the microorganism; inorganic substances in the culture medium and a malt extract provide nutrition for the microorganism; the temperature of 30-37 DEG C of the culture medium is optimally suitable for culture of the microorganism; the cell walls are broken during cell centrifugation, so as to facilitate the follow-up reactions; an ATP releasing agent can help the microorganism perform a series of coupled reactions; at last the added luciferase enables the microorganism to emit light, so as to help identify whether the sample contains the microorganism or not.
Description
technical field [0001] The invention relates to the field of microorganism detection, in particular to a method for detecting microorganisms. Background technique [0002] At present, the detection of the number of bacteria, mold and yeast, which is the most common routine detection item for microorganisms, still uses the agar plate counting method established by Pasteur more than 100 years ago. Traditional microbial detection methods rely on special microbial culture media to isolate and culture the living microorganisms in samples. These methods are sensitive and intuitive, and can provide information on the number and types of microorganisms in food at the same time. However, the conventional method takes 1 to 5 days from the beginning of cultivation to the colonies visible to the naked eye, and the operation is cumbersome, limited to the laboratory, requiring high technical level of the operator, and prone to human errors; It is generally believed that only 5-10% of m...
Claims
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IPC IPC(8): C12Q1/66C12Q1/02
Inventor 王之侃孙文勇
Owner 安徽信灵检验医学科技股份有限公司
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