Rapid Propagation Method of White Uncaria

A kind of white Uncaria, fast technology, applied in the field of rapid reproduction of white Uncaria, to achieve the effect of improving tissue cell division, promoting formation, and accelerating growth rate

Active Publication Date: 2017-03-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resources of Uncaria vulgaris are on the verge of exhaustion, so the introduction and cultivation and artificial propagation will become new ways to effectively utilize the resources of Uncaria vulgaris. At present, there are no research reports on the cultivation and reproduction of Uncaria vulgaris.

Method used

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  • Rapid Propagation Method of White Uncaria
  • Rapid Propagation Method of White Uncaria
  • Rapid Propagation Method of White Uncaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of rapid propagation method of white hook vine, comprising the following steps:

[0036] Step 1. Make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 2cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 25min, then continue to rinse the explant with sterilized tea water 20s.

[0037]Step 2, transfer the sterile explants into the first culture medium for li...

Embodiment 2

[0048] A kind of rapid propagation method of white hook vine, comprising the following steps:

[0049] Step 1. To make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove the excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 4cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 30min, then continue to rinse the explant with sterilized tea water 30s.

[0050] Step 2. Transfer the sterile explants into the first culture mediu...

Embodiment 3

[0061] A kind of rapid propagation method of white hook vine, comprising the following steps:

[0062] Step 1. To make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 3cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 26 minutes, then continue to rinse the explant with sterilized tea water 26s.

[0063] Step 2, transfer the sterile explants into the first culture medi...

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Abstract

The invention discloses a rapid propagation method of Uncaria sessilifructus Roxb.. The rapid propagation method comprises the following steps: step 1, manufacturing an aseptic explant of Uncaria sessilifructus Roxb.; step 2, carrying out illumination culturing on the aseptic explant in a first culture medium; step 3, carrying out illumination culturing on the aseptic explant with axillary buds obtained in the step 2 in a second culture medium till roots grow out; and step 4, transplanting the rooted Uncaria sessilifructus Roxb. seedling into a medium for culturing. According to the rapid propagation method established in the invention, the propagation rate is high, the propagation is rapid, large-area popularization and planting in production are facilitated, and the urgent needs for development of natural resources of Chinese medicinal materials are met.

Description

technical field [0001] The invention relates to the field of plant cultivation, and more specifically, the invention relates to a method for rapid propagation of white hook vine. Background technique [0002] Uncaria uncaria, also known as Uncaria sessileus, is distributed in Guangdong, Guangxi, Yunnan and other places. It is born in dense forests or forest valley bushes. The utilization of white hook vine is mainly based on wild resources. At present, there are also a small amount of introduced cultivation. The cultivation is mainly based on seed sowing and cutting propagation, but the survival rate and reproduction coefficient are low. The use of tissue culture rapid propagation technology can effectively and quickly increase Its reproduction coefficient can not only meet the market demand for white uncaria medicinal materials, but also benefit the protection of wild resources of white uncaria. At present, the tissue rapid propagation of other species in the genus Uncaria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韦树根马小军白隆华潘丽梅付金娥
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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