Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicinal use of a kind of lycorine and bortezomib composition

A technology of bortezomib and lycorine, which is applied in the field of new pharmaceutical uses of lycorine and bortezomib compositions, can solve problems such as drug resistance, and achieve the effect of improving sensitivity, inhibiting cell autophagy, and improving cell proliferation. Effect

Active Publication Date: 2018-09-21
CENT SOUTH UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use of bortezomib, myeloma cells are resistant to bortezomib clinically.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicinal use of a kind of lycorine and bortezomib composition
  • Medicinal use of a kind of lycorine and bortezomib composition
  • Medicinal use of a kind of lycorine and bortezomib composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: In this example, the CCK-8 method was used to detect the effects of lycorine hydrochloride and bortezomib on the survival rate of MM cells after single and combined use.

[0020] Experimental steps:

[0021] (1) Collect ARH-77 and ANBL-6 cells in the logarithmic growth phase, adjust the concentration of the cell suspension, add 100 μL to each well, and plate on a 96-well plate to make the cell density to be tested 2×10 5 a / mL;

[0022] (2) Set up a zero-adjustment group without adding cells and only medium, and a DMSO control group without adding drugs at an appropriate concentration, and set 3-5 parallel wells for each concentration;

[0023] (3) Add 10 μM lycorine hydrochloride, 15 nM bortezomib and 10 μM lycorine hydrochloride and 15 nM bortezomib respectively, and place in 5% CO 2 , incubate in a cell culture incubator at 37°C for 24 hours;

[0024] (4) Add 10 μL of CCK-8 solution to each well, incubate at 37°C in the dark for 2 hours, measure the OD v...

Embodiment 2

[0026] Example 2: In this example, the CCK-8 method was used to detect the synergistic effect on MM cells after combined use of different concentration ratios of lycorine hydrochloride and bortezomib on MM cells.

[0027] Experimental steps:

[0028] (1) Collect ARH-77 and ANBL-6 cells in the logarithmic growth phase, adjust the concentration of the cell suspension, add 100 μL to each well, and plate on a 96-well plate to make the cell density to be tested 2×10 5 a / mL;

[0029] (2) Set up a zero-adjustment group without adding cells and only medium, and a DMSO control group without adding drugs at an appropriate concentration, and set 3-5 parallel wells for each concentration;

[0030] (3) Add different concentration ratios of lycorine hydrochloride and bortezomib (2.5μM+5nM, 2.5μM+10nM, 2.5μM+15nM, 5μM+5nM, 5μM+10nM, 5μM+15nM, 10μM+5nM , 10μM+10nM or 10μM+15nM), placed in 5% CO 2 , incubate in a 37°C cell culture incubator for 24 hours;

[0031] (4) Add 10 μL of CCK-8 sol...

Embodiment 3

[0033] Example 3: In this example, the Western Blot method was used to detect the effects of lycorine hydrochloride and bortezomib on the autophagy-related proteins LC3B and Beclin1 after single and combined use on ARH-77 and ANBL-6 cells.

[0034] Experimental steps:

[0035] (1) Collect ARH-77 and ANBL-6 cells in logarithmic growth phase, adjust the concentration of cell suspension to 5×10 5 cells / mL in a cell culture flask with a total volume of 10 mL, and then add 10 μM lycorine hydrochloride, 15 nM bortezomib, and 10 μM lycorine hydrochloride and 15 nM bortezomib in combination, and use the corresponding concentration of DMSO as a control group. Place in a cell culture incubator and incubate for 24 hours;

[0036] (2) Collect the cells, wash twice with 5mL PBS, add an appropriate volume of RIPA cell lysate (containing 0.5% protease inhibitor cocktail) to each tube, mix well, lyse on ice for 30 minutes, sonicate on ice for 30 seconds, 4°C Centrifuge for 15 minutes, aspir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides new application for a lycorine and bortezomib composition medicine. The lycorine and bortezomib composition is used for preparing a medicinal preparation for treating multiple myeloma. Compared with the fact that lycorine and bortezomib are used separately, combined use of lycorine and bortezomib can realize a better cell proliferation inhibition effect, and cell autophagy can be inhibited through combined use. The composition greatly improves sensibility of multiple myeloma to bortezomib and is applicable to drug-resistant multiple myeloma which is difficult to treat.

Description

technical field [0001] The present invention relates to the technical field of the composition of lycorine and bortezomib, and more specifically relates to a new drug application of the composition of lycorine and bortezomib. Background technique [0002] Multiple myeloma (multiple myeloma, MM) is a malignant tumor derived from terminally differentiated B lymphocytes. It is characterized by the clonal proliferation of a large number of plasma cells in the bone marrow. MM is still incurable. The bone marrow microenvironment is closely related to the proliferation, migration, survival, drug resistance and bone disease of MM. The nuclear factor NF-κB signaling pathway is a transcriptional regulator that plays an important role in the pathogenesis of MM. Bone marrow stromal cells HS-5 cells can produce GM-CSF, M-CSF, LIF and other cytokines to promote the proliferation and drug resistance of MM, so HS-5 cells can well simulate the bone marrow microenvironment of MM in vitro. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/05A61K31/69A61P35/00A61K31/4745
Inventor 刘静萧小鹃周文梁龙罗伊陈慧勇
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com