Extraction and purification method of 2 alpha-hydroxytriptonide

A purification method and lactone ketone technology, applied in the field of traditional Chinese medicine research, can solve problems such as being highly toxic and failing to be widely promoted

Inactive Publication Date: 2015-12-30
FUJIAN ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing away heat and detoxification, reducing swelling and dissipating stagnation, expelling wind and dampness

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] a 2 α - the extraction and purification method of hydroxy triptolide, which may further comprise the steps:

[0027] 1) Crush 10kg of the root core, root bark, stem and leaves of Tripterygium wilfordii, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 24 hours, then use 30 times the weight of tripterygium in ethanol for percolation. Collect the filtrate once every 500mL, concentrate to dryness, add 1mL of chloroform to dissolve it, and use TLC to detect it qualitatively, then combine and concentrate the percolation liquid to obtain the ethanol concentrate;

[0028] 2) Extract the obtained ethanol concentrate with ethyl acetate at a volume ratio of 1:1.5. During each extraction process, it needs to be shaken 20 times. After each extraction, the collected ethyl acetate solution is concentrated and analyzed by TLC. Qualitative detection, after extraction for 3 times, the extracted ethyl acetate solution was combined, concentrated, and dried by ...

Embodiment 2

[0033] a 2 α - the extraction and purification method of hydroxy triptolide, which may further comprise the steps:

[0034] 1) Crush 10kg of the root core, root bark, stem and leaves of Tripterygium wilfordii, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 24 hours, then use 32 times the weight of tripterygium in ethanol for percolation. Collect the filtrate once every 500mL, concentrate to dryness, add 1mL of chloroform to dissolve it, and use TLC to detect it qualitatively, then combine and concentrate the percolation liquid to obtain the ethanol concentrate;

[0035]2) Extract the obtained ethanol concentrate with ethyl acetate at a volume ratio of 1:1.5. During each extraction process, it needs to be shaken 20 times. After each extraction, the collected ethyl acetate solution is concentrated and analyzed by TLC. Qualitative detection, after 5 times of extraction, the extracted ethyl acetate solutions were combined, concentrated, and dried by ...

Embodiment 3

[0040] a 2 α - the extraction and purification method of hydroxy triptolide, which may further comprise the steps:

[0041] 1) Crush 10kg of the root core, root bark, stem and leaves of Tripterygium wilfordii, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 24 hours, then use 35 times the weight of tripterygium in ethanol for percolation. Collect the filtrate once every 500mL, concentrate to dryness, add 1mL of chloroform to dissolve it, and use TLC to detect it qualitatively, then combine and concentrate the percolation liquid to obtain the ethanol concentrate;

[0042] 2) Extract the obtained ethanol concentrate with ethyl acetate at a volume ratio of 1:1.5. During each extraction process, it needs to be shaken 20 times. After each extraction, the collected ethyl acetate solution is concentrated and analyzed by TLC. Qualitative detection, after 6 times of extraction, the extracted ethyl acetate solution was combined, concentrated, and dried by m...

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PUM

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Abstract

The invention discloses an extraction and purification method of 2 alpha-hydroxytriptonide. By using root cores, root barks, stems and leaves of Tripterygium willfordii Hook. f as raw materials, through steps such as crushing, soaking, percolation, extraction, silica-gel column chromatography separation and purification and recrystallization, 2alpha-hydroxytriptonide with purity which is above 99 percent can be prepared, the yield is one ten thousandth and is improved by about 100 times compared with the yield by adopting extraction processes reported by the existing literatures, and a material basis can be provided for researches on chemical structures, pharmacological actions and the like of triptonide.

Description

technical field [0001] The invention belongs to the technical field of Chinese medicine research, in particular to a 2 α -Extraction and purification method of hydroxy triptolide. Background technique [0002] Tripterygium ( Tripterygium willfordii Hook.f) is a plant of the genus Tripterygium wilfordii in the family Euonymus, which is used as medicine with the whole root or the xylem of the peeled root. Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing heat and detoxification, reducing swelling and dissipating stagnation, expelling wind and dampness, killing insects and stopping bleeding. It is highly toxic, and its clinically effective dose is close to the poisoning dose, so the application of tripterygium wilfordii has not been widely promoted so far. [0003] In order to give full play to the medicinal efficacy of Tripterygium wilfordii, domestic and foreign scholars have isolated dozens of chemical components fro...

Claims

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Application Information

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IPC IPC(8): C07J73/00
Inventor 林绥阙慧卿彭华毅黄郑隽郭舜民钱丽萍
Owner FUJIAN ACAD OF MEDICAL SCI
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