A strain of Bacillus amyloliquefaciens bam22 with high efficiency in controlling clubroot of cruciferous species

A technology of amylolytic spores and Brassicaceae, applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems of poor control effects of biocontrol strains, unfavorable balance of soil flora, and unsatisfactory control effects, etc., to achieve good results Inhibitory effect, low application concentration, and low application dose

Active Publication Date: 2019-03-22
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, although some progress has been made in the research of biocontrol strains, there are still at least three problems: (1) The control effect of biocontrol strains alone is not good, and they often need to be used in combination with chemical agents, which limits The further application of biocontrol strains in the prevention and treatment of cruciferous clubroot; (2) The large application dose is not conducive to the balance of soil flora. If it is used for a long time, it may have unpredictable effects; (3) Due to clubroot Bacteria cannot be cultured in vitro, so there is no definite theory for the efficient development of clubroot biocontrol strains, so few strains have been developed. Although some bacillus are known to be effective in the control of clubroot, some have been developed. There are no more than 10 Bacillus strains with certain effects, and the main ones are Bacillus subtilis, and the control effect is not satisfactory.

Method used

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  • A strain of Bacillus amyloliquefaciens bam22 with high efficiency in controlling clubroot of cruciferous species
  • A strain of Bacillus amyloliquefaciens bam22 with high efficiency in controlling clubroot of cruciferous species
  • A strain of Bacillus amyloliquefaciens bam22 with high efficiency in controlling clubroot of cruciferous species

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Control effect experiment of Bacillus amyloliquefaciens Bam22 on cruciferous clubroot

[0018] Experimental objects: Rapeseed, Chinese cabbage, broccoli.

[0019] After culturing Bacillus amyloliquefaciens Bam22 for 48 hours, dilute it 200 times, 500 times, 800 times, and 1000 times, and use the root watering method to water 100ml of each plant when transplanting, once every 7 days, and then again every 14 days . Note: The bacterial concentration of the undiluted bacterial solution is about 6-8×10 9 cfu / ml.

[0020] Disease classification:

[0021] Grade 0: There is no tumor in the root, and the root system develops normally;

[0022] Grade 1: 1-30% of the roots have a little tumor, with small nodular masses that are not connected to each other;

[0023] Grade 2: 31-60% of root tumors with large nodular or globular masses, interconnected;

[0024] Grade 3: 61-100% root tumors, enlarged to form spindle-shaped tumors, large tumor masses extending to hypocotyls, pla...

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Abstract

The invention provides efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22. The efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22 is preserved in China General Microbiological Culture Collection Center on September 6, 2015, and a preservation number of the bacillus amyloliquefaciens is CGMCC No.11329. The strain has an efficient disease prevention effect on crucifer club root, has low concentration of applied bacterium liquid and less dosage, and has a very great actual application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a high-efficiency cruciferous clubroot biocontrol bacillus amyloliquefaciens Bam22. Background technique [0002] Brassicaceae clubroot is a worldwide disease caused by Plasmodium brassicae infection. It is spreading in Europe, the United States, Canada, Japan and other countries and regions, seriously threatening the production of Brassicaceae crops. [0003] Cruciferous clubroot in China mainly occurs in East China and South China. Since the 1990s, cruciferous clubroot has spread to Sichuan. Today, the economic losses caused by the clubroot disease in Chengdu only in cruciferous crops have reached hundreds of millions of RMB per year. In addition, rape, as an important oil crop, is facing a particularly serious threat. Therefore, it is necessary to find effective methods to prevent and control clubroot in cruciferous plants (especially rapeseed). ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 黄小琴刘勇张蕾伍文宪周西全刘红雨
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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